Mesenchymal Cell News Volume 7.23 | Jun 16 2015

Simple Coating with Fibronectin Fragment Enhances Stainless Steel Screw Osseointegration in Healthy and Osteoporotic Rats
The authors present a simple coating strategy based on passive adsorption of FN7-10, a recombinant fragment of human fibronectin encompassing the major cell adhesive, integrin-binding site, onto 316-grade stainless steel (SS). FN7-10 coating on SS surfaces promoted α5β1 integrin-dependent adhesion and osteogenic differentiation of human mesenchymal stem cells. [Biomaterials] Abstract

SWI/SNF-Mediated Lineage Determination in Mesenchymal Stem Cells Confers Resistance to Osteoporosis
BRM is not required for development, which has hindered understanding of its contributions, but knockdown genetics, designed to explore the hypothesis that BRM-SWI/SNF has different regulatory roles in different mesenchymal stem cell lineages, showed that depleting BRM from mesenchymal stem cells has a dramatic effect on the balance of lineage selection between osteoblasts and adipocytes. [Stem Cells] Abstract

A Method for Performing Islet Transplantation Using Tissue-Engineered Sheets of Islets and Mesenchymal Stem Cells
Investigators describe a tissue engineering approach using islets with mesenchymal stem cell sheets to improve the therapeutic effect of islet transplantation. [Tissue Eng Part C Methods] Abstract

Gata4, Tbx5 and Baf60c Induce Differentiation of Adipose Tissue-Derived Mesenchymal Stem Cells into Beating Cardiomyocytes
Scientists showed that the combination of Gata4, Tbx5 and Baf60c is sufficient for inducing adipose tissue-derived mesenchymal stem cells to form cardiomyocytes. It also appears that, while Gata4 and Baf60c are key inducers of myocardial differentiation, Tbx5 is essential for the ability of cardiac cells to contract. [Int J Biochem Cell Biol] Abstract

Human Adipose Tissue-Derived Mesenchymal Stem Cells Target Brain Tumor-Initiating Cells
Both in vitro and in vivo experiments showed that human adipose tissue derived mesenchymal stem cells possess migratory abilities to target brain tumor-initiating cells isolated from medulloblastoma, atypical teratoid/rhabdoid tumors and glioblastoma. [PLoS One] Full Article

Connexin43 Mediated Delivery of ADAMTS5 Targeting siRNAs from Mesenchymal Stem Cells to Synovial Fibroblasts
Researchers transduced human mesenchymal stem cells (hMSCs) with ADAMTS5-targeting shRNA or non-targeted shRNA, and co-cultured them with synovial fibroblasts to allow delivery of siRNAs from hMSC to synovial fibroblasts. They found that co-culture of hMSCs-shRNA-ADAMTS5 and synovial fibroblasts reduced ADAMTS5 expression relative to co-culture of hMSCs-shRNA-control and synovial fibroblasts. [PLoS One] Full Article

Effect of Bioactive Glass Particles on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells Seeded on Lactide and Caprolactone Based Scaffolds
In this study the influence of incorporating bioactive glass particles to lactide and caprolactone based porous scaffolds on osteogenic differentiation of adipose-derived stem cells was analyzed. [J Biomed Mater Res A] Abstract

Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes
Scientists used immortalized human bone marrow stromal cells (BMSC) clonal lines for multi-level analysis of functional markers for BMSC subsets. All clones expressed typical BMSC cell-surface antigens; however, clones with trilineage differentiation capacity exhibited enhanced vascular interaction gene sets, whereas non-differentiating clones were uniquely CD317 positive with significantly enriched immunomodulatory transcriptional networks and high IL-7 production. [Stem Cell Rep] Full Article | Press Release

Mesenchymal Stem Cells and Adaptive Immune Responses
The authors review the influence of mesenchymal stem cells (MSCs) on adaptive immune system, especially their bidirectional interaction in tuning the immune microenvironment and subsequently repairing damaged tissue. Understanding MSC-mediated regulation of T cells is expected to provide fundamental information for guiding appropriate applications of MSCs in clinical settings. [Immunol Lett] Abstract

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Cord Blood Registry’s New Disease Registry Brings Data & Families Together to Advance Stem Cell Research
Cord Blood Registry^® announced the launch of CBR’s Family Health Registry, a unique registry designed to offer insight into common diseases and conditions affecting families and to bring patients, healthcare professionals and researchers together to expedite and facilitate research related to the discovery of stem cell treatments and potential cures. [Cord Blood Registry^®] Press Release

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Scientist – Myogenic Progenitor Cell Biology (STEMCELL Technologies Inc.)

Research Scientist – Stem Cell Biology (ODU Research Foundation)

Postdoctoral Fellowship – Molecular Biology and Epigenetics (Rutgers New Jersey Medical School)

PhD Positions – Cartilage Regeneration in Joints and Intervertebral Disc Diseases (Cardiff University)

Assistant/Associate/Full Professor – Stem Cells (Shanghai Jiao Tong University)

Postdoctoral Fellow – Stem Cell Biology and Pharmacogenomics (Northwestern University)

Postdoctoral Position – Stem Cells (Technion – Israel Institute of Technology)

Postdoctoral Fellow/Research Associate – Leukemia, Stem Cells and Development (Josep Carreras Leukemia Research Institute)

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