A Computer-Designed Scaffold for Bone Regeneration within Cranial Defect Using Human Dental Pulp Stem Cells Researchers used a computer-designed, solvent-free scaffold and human dental pulp stem cells (hDPSCs) to regenerate neo-bone within cranial bone defects. The hDPSCs expressed mesenchymal stem cell markers and served as an abundant source of stem cells with a high proliferation rate. [Sci Rep] Full Article Mesenchymal Stem Cell Expression of SDF-1β Synergizes with BMP-2 to Augment Cell-Mediated Healing of Critical-Sized Mouse Calvarial Defects The authors tested the hypothesis that stromal cell-derived factor-1β (SDF-1β) overexpressed in Tet-Off-SDF-1β bone marrow-derived mesenchymal stem/stromal cells (BMSCs), delivered on acellular dermal matrix, synergistically augments bone morphogenetic protein-2 (BMP-2)-induced healing of critical-sized mouse calvarial defects. BMSC therapies alone showed limited bone healing, which was increased with co-delivery of BMP-2. [J Tissue Eng Regen Med] Abstract Growth Factors Cross-Linked to Collagen Microcarriers Promote Expansion and Chondrogenic Differentiation of Human Mesenchymal Stem Cells Collagen microcarriers cross-linked with basic fibroblast growth factor or transforming growth factor-β1 were used for expansion and chondrogenic differentiation of human mesenchymal stem cells. Evaluation methods included cell viability test, chondrogenic marker expression, histological detection of proteoglycans and immunohistochemical analysis. [Tissue Eng Part A] Abstract Mouse White Adipose Tissue-Derived Mesenchymal Stem Cells Gain Pericentral and Periportal Hepatocyte Features after Differentiation In Vitro, which Are Preserved In Vivo after Hepatic Transplantation Adipose tissue-derived mesenchymal stem cells were isolated from immunodeficient C57BL/6 mice and differentiated into the hepatocytic phenotype by applying a simple protocol. Their physiological and metabolic functions were analyzed in vitro and after hepatic transplantation in vivo. [Acta Physiol] Abstract Differential Expression of Adhesion-Related Proteins and MAPK Pathways Leads to Suitable Osteoblast Differentiation of Human Mesenchymal Stem Cells Subpopulations Investigators analyzed the expression profiles of adhesion-related proteins during osteoblastic differentiation of two human mesenchymal stem cells subpopulations and proposed a strategy for assembling bone grafts based on its adhesion ability. [Stem Cells Dev] Abstract Effects of Tanshinone IIA on Osteogenic Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells Both ALP activity at day 7 and calcium content at day 24 were upregulated during the osteogenesis of mouse bone marrow mesenchymal stem cells treated with tanshinone IIA (TSA), demonstrating that it promoted the osteogenesis at both early and late stages. They found that TSA promoted osteogenesis and inhibited osteoclastogenesis, evident by RT-PCR analysis of osteogenic marker gene expressions. [Naunyn Schmiedebergs Arch Pharmacol] Abstract Osteogenic Potential of In Vitro Osteo-Induced Adipose-Derived Mesenchymal Stem Cells Combined with Platelet-Rich Plasma in an Ectopic Model The osteogenic potential of in vitro osteo-induced adipose-derived mesenchymal stem cells combined with platelet-rich plasma and implanted on bone mineral matrix carrier was examined in a subcutaneous model in Balb/c mice. [Int Orthop] Abstract Adipose-Derived Mesenchymal Stem Cells Ameliorate Ulcerative Colitis through miR-1236 Negatively Regulating the Expression of Retinoid-Related Orphan Receptor Gamma The authors investigated the effect of adipose-derived mesenchymal stem cells on 2,4,6-trinitrobenzenesulfonic acid-induced ulcerative colitis in rat colon tissues. qRT-PCR and immunofluorescence analyses were performed to monitor the expression of miR-1236 and its target molecule, retinoid-related orphan receptor γ. [DNA Cell Biol] Abstract Effects of Angular Frequency during Clinorotation on Mesenchymal Stem Cell Morphology and Migration Scientists investigated the effects of clinorotation speed on the morphology, cytoarchitecture, and migration behavior of human mesenchymal stem cells. [NPJ Microgravity] Full Article |