IN VITRO Hypoxia Created Human Mesenchymal Stem Cell Sheet for Prevascularized 3D Tissue Construction Scientists investigated the vascularization of human mesenchymal stem cell (hMSC) sheets under different oxygen tensions. They found that the HN condition, in which hMSC sheets formed under physiological hypoxia and then cocultured with endothelial cells under normoxia, enables longer and more branched microvessel network formation. [Adv Healthc Mater] Abstract Simple Surface Engineering of Polydimethylsiloxane with Polydopamine for Stabilized Mesenchymal Stem Cell Adhesion and Multipotency The authors aimed to tailor greener and biocompatible polydimethylsiloxane (PDMS) surfaces by developing a one-step bio-inspired polydopamine coating strategy to stabilize long-term bone marrow stromal cell culture on PDMS substrates. [Sci Rep] Full Article Comparison of the Depolarization Response of Human Mesenchymal Stem Cells from Different Donors Human mesenchymal stem cells from five donors were evaluated for their response to membrane potential depolarization. Scientists compared the inter-donor variability of their osteogenic and adipogenic differentiation potential, as well as their ability to maintain a differentiated phenotype after induction. [Sci Rep] Full Article Genetic Modification of Human Mesenchymal Stem Cells Helps to Reduce Adiposity and Improve Glucose Tolerance in an Obese Diabetic Mouse Model Researchers studied how high glucose (HG) exposure affects mesenchymal stem cells (MSCs) differentiation and whether the mechanism is reversible. They exposed human adipose tissue derived MSCs to HG and compared it to normal glucose exposed cells at 7, 10 and 14 days. [Stem Cell Res Ther] Full Article Knockdown of SIRT6 Enables Human Bone Marrow Mesenchymal Stem Cell Senescence Sirtuin 6 (SIRT6) expression increased with age while the proliferation and migration abilities of aged human bone marrow mesenchymal stem cells (BM-MSCs) were decreased compared with young cells. Knockdown of SIRT6 impaired the proliferative, migration, and oxidative stress resisting potentials of BM-MSCs. [Rejuvenation Res] Abstract Collagen Promotes Higher Adhesion, Survival and Proliferation of Mesenchymal Stem Cells Scientists studied the effect of extracellular matrix proteins in controlling cell survival, proliferation, migration and directed mesenchymal stem cell differentiation. They found that collagen promoted cell proliferation, cell survival under stress and promoted high cell adhesion to the cell culture surface. [PLoS One] Full Article Evaluation of Cytocompatibility of Calcium Silicate-Based Endodontic Sealers and Their Effects on the Biological Responses of Mesenchymal Dental Stem Cells Researchers investigated in vitro the cytocompatibility of the calcium silicate-containing endodontic sealers mineral trioxide aggregate Fillapex and TotalFill BC Sealer in human periodontal ligament stem cells by assaying their biological responses and compared them with that observed when using an epoxy resin-based sealer. [Int Endod J] Abstract IN VIVO A Distinct Subpopulation of Bone Marrow Mesenchymal Stem Cells, Muse Cells, Directly Commit to the Replacement of Liver Components Bone marrow mesenchymal stem cells (BM-MSCs) have been suggested to contribute to liver regeneration but compose a heterogeneous population. Investigators generated a physical partial hepatectomy model in immunodeficient mice and injected green fluorescent protein-labeled human BM-MSC multilineage-differentiating stress-enduring (muse) cells intravenously. [Am J Transplant] Abstract Effects of Mesenchymal Stem Cell Treatment on the Expression of Matrix Metalloproteinases and Angiogenesis during Ischemic Stroke Recovery Scientists investigated the optimal time point of mesenchymal stem cell (MSC) transplantation. After MSC treatment, they also investigated the expression of matrix metalloproteinases, which play a role in vascular and tissue remodeling. [PLoS One] Full Article |