Mesenchymal Cell News Volume 8.03 | Jan 26 2016

A Single Short Session of Media Perfusion Induces Osteogenesis in hBMSCs Cultured in Porous Scaffolds, Dependent on Cell Differentiation Stage
Scientists investigated whether a short, single perfusion session applied to human bone-marrow derived mesenchymal stem cells (hBMSCs) cultured in 3D would enhance their osteogenesis in vitro. [Biotechnol Bioeng] Abstract

Effect of Nano-HA/Collagen Composite Hydrogels on Osteogenic Behavior of Mesenchymal Stromal Cells
Researchers evaluated the in vitro effect of nanosized hydroxyapatite (HA) and collagen based composite hydrogels on the behavior of human mesenchymal stromal cells (MSCs), isolated from either adipose tissue or bone marrow. [Stem Cell Rev] Full Article

Shear Stress and Circumferential Stretch by Pulsatile Flow Direct Vascular Endothelial Lineage Commitment of Mesenchymal Stem Cells in Engineered Blood Vessels
The appropriate biomechanical stimulation conditions for differentiating mesenchymal stem cells (MSCs) into ECs have not been fully investigated. To accomplish an in vivo-like loading environment, a loading system was designed to apply flow induced stress and induce hMSC differentiation into vascular cells. [J Mater Sci Mater Med] Abstract

Inhibition of miR-222-3p Activity Promoted Osteogenic Differentiation of hBMSCs by Regulating Smad5-RUNX2 Signal Axis
Researchers identified that miR-222-3p decreased during osteogenic differentiation of human mesenchymal stem cells (hBMSCs) using quantitative real-time reverse transcription PCR. Furthermore, they investigated the effect of miR-222-3p on osteogenic differentiation of hBMSCs. [Biochem Biophys Res Commun] Abstract

IN VIVO

Microbubble-Mediated Ultrasound Promotes Accumulation of Bone Marrow Mesenchymal Stem Cell to the Prostate for Treating Chronic Bacterial Prostatitis in Rats
A model of chronic bacterial prostatitis was established in SD rats, which were then treated with bone marrow mesenchymal stem cells (BMMSCs) alone, BMMSCs with ultrasound, BMMSCs with microbubble-mediated ultrasound and compared with a healthy control group. [Sci Rep] Full Article

Overexpression of Protein Kinase C ε Improves Retention and Survival of Transplanted Mesenchymal Stem Cells in Rat Acute Myocardial Infarction
Investigators assessed the effects of protein kinase C ε (PKCε) for improving stem cell therapy for acute myocardial infarction (AMI). Primary mesenchymal stem cells (MSCs) were harvested from rat bone marrow. PKCε-overexpressed MSCs and control MSCs were transplanted into infarct border zones in a rat AMI model. [Cell Death Dis] Full Article

Mesenchymal Stem Cells Alleviate LPS-Induced Acute Lung Injury in Mice by MiR-142a-5p-Controlled Pulmonary Endothelial Cell Autophagy
The authors found that mesenchymal stem cells (MSCs) transplantation significantly reduced the severity of lipopolysaccharide (LPS)-induced acute lung injury in mice. MSCs increased autophagy of pulmonary endothelial cells (PECs) to promote PEC survival. [Cell Physiol Biochem] Full Article

Transplantation of CXCR4 Overexpressed Mesenchymal Stem Cells Augments Regeneration in Degenerated Intervertebral Discs
The authors evaluated whether high expression of CXCR4 enhances the migration of mesenchymal stem cells and increases the efficiency of intervertebral disc regeneration. [DNA Cell Biol] Abstract

P53 Functional Abnormality in Mesenchymal Stem Cells Promotes Osteosarcoma Development
In the context of loss of function of p53, the authors provide a model for two sources of osteosarcoma: mesenchymal stem cells as progenitor cells of osteoblasts and bone tumor microenvironment components. [Cell Death Dis] Full Article

Mesenchymal Stem Cells and Immunomodulation: Current Status and Future Prospects
Investigators summarize the preclinical and clinical studies of mesenchymal stem cells (MSCs) from different adult tissues, discuss the current hurdles to their use and propose the future development of pluripotent stem cell-derived MSCs as an approach to immunomodulation therapy. [Cell Death Dis] Full Article

Current Clinical Evidence for the Use of Mesenchymal Stem Cells in Articular Cartilage Repair
Scientists provide an overview of the clinical evidence on the use of mesenchymal stem cells in articular cartilage repair. [Expert Opin Biol Ther] Abstract

Visit our reviews page to see a complete list of reviews in the mesenchymal cell research field.

Center Will Develop Consistent Manufacturing Processes for Cell-Based Therapies
A $15.7 million grant from the Marcus Foundation has helped launch a new Georgia Institute of Technology research center that will develop processes and techniques for ensuring the consistent, low-cost, large-scale manufacture of high-quality living cells used in cell-based therapies. [Georgia Institute of Technology] Press Release

From our sponsor:
Learn about mucociliary differentiation of primary bronchial epithelial cells. Watch the video.

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

Visit our events page to see a complete list of events in the mesenchymal cell community.

Tenure-Track Faculty Positions – Assistant/Associate/Full Professor (Nanyang Technological University)

Postdoctoral Positions – Regenerative Biology and Medicine (Helmholtz Zentrum München)

Development Engineer/Scientist – Cell and Gene Therapy and Regenerative Medicine (Centre for Commercialization of Regenerative Medicine)

Project Manager – Neurodegeneration and Stem Cell Science (Université du Luxembourg)

Postdoctoral Research Associate – Developmental and Stem Cell Biology (University of Cambridge)

Postdoctoral Fellow – Cell Therapy (Toronto Western Hospital)

Stem Cell Scientist and Postdoctoral Positions (National Center for Advancing Translational Science)

PhD Studentship – Aging Research (Cologne Graduate School of Aging Research)

Doctoral Fellow – Stem Cell Research (Uniklinik Koln)

Postdoctoral Fellow – Stem Cell Biology/Genome Engineering (Amgen)

Recruit Top Talent: Reach more than 60,000 potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.