Mesenchymal Cell News 9.27 July 11, 2017 | |
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TOP STORYInvestigators fabricated four millimeter inner diameter vascular tissues using mesenchymal progenitor cells seeded into tubular scaffolds. Their data demonstrated that pulsatile forces and perfusion of 3D tubular constructs from both the lumenal and ablumenal sides with culture media significantly improved tissue assembly, effectively inducing mesenchymal progenitor cell differentiation to smooth muscle cells with contemporaneous elastin production. [Acta Biomater] Abstract | Graphical Abstract | |
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PUBLICATIONS(Ranked by impact factor of the journal)IN VITROResearchers investigated the impact of histone deacetylase 1 (HDAC1) on cardiac mesenchymal cell (CMC) cytokine secretion and associated paracrine-mediated activities on endothelial cell function. After evaluating key cytokines previously implicated in cell-therapy-mediated cardiac repair, they found that basic fibroblast growth factor was significantly upregulated in shRNA constructs targeting HDAC1-transduced CMCs. [J Am Heart Assoc] Full Article The authors aimed to evaluate the in vitro effects of equine MSCs on the growth of representative gram-negative and gram-positive bacterial species commonly found in skin wounds and define the mechanisms by which MSCs inhibit bacterial growth. They found that equine MSCs and MSC conditioned medium (CM) inhibited the growth of E. coli and S. aureus, and that MSC CM depolarizes the cell membranes of these bacteria. [Stem Cell Res Ther] Full Article | Press Release Researchers present a designer self-assembling peptide, D-RADA16, made of all D-amino acid. They investigated the nanofiber morphology of D-RADA16, its potential for the culture of bone marrow-derived mesenchymal stem cells (BMSCs), and the proteolytic resistance of the biomaterial. D-RADA16 and L-RADA16 hydrogel scaffolds were both found to promote the proliferation and migration of rat BMSCs in the 3D cell culture microenvironment. [Int J Mol Med] Abstract | Full Article (Download) Scientists provide the development and characterization of custom-made spray nozzles that could serve as a foundation for the development of a BioAirbrush, a platform technology for the arthroscopic application of (cell-laden) hydrogels. They evaluated the cell survival of chondrocytes and MSCs as well as the chondrogenic capacity of chondrocytes after spraying. [Tissue Eng Part C Methods] Abstract IN VIVOOn the basis of CD29 and CD54 expression, investigators prospectively isolated a population with a high colony-forming ability and multi-lineage potential from the rat bone marrow, and they demonstrated that most of these cells expressed CD73. Successful engraftment of the rat MSCs was achieved using a fluorescence-conjugated anti-CD73 antibody. [Sci Rep] Full Article Researchers reprogrammed bone marrow-derived MSCs into insulin-producing cells via chicken ovalbumin upstream promoter transcriptional factor I suppression and MafA overexpression. The differentiated cells expressed higher levels of genes specific for islet endocrine cells, and they released C-peptide and insulin in response to glucose stimulation. [Mol Ther Nucleic Acids] Full Article Periostin Promotes Ectopic Osteogenesis of CTLA4-Modified Bone Marrow Mesenchymal Stem Cells The extracellular matrix (ECM) has recently been reported to play a vital role in bone formation and periostin (POSTN) has been suggested as a key member in constructing the ECM in bone tissue. The authors found that POSTN expression in a 4-Ig-modified bone marrow mesenchymal stem cell (MSCs-CTLA4) group was significantly enhanced compared with that in the MSCs group, not only in tissue-engineered bone with femur heterotopic transplantation in vivo but also under the immune activation condition in vitro. [Cell Tissue Res] Abstract Investigators combined MSCs and ex vivo interleukin (IL)-10 gene transfer with an adeno-associated virus vector using a rat transient middle cerebral artery occlusion model. Immunological analysis showed that MSC/IL-10 transplantation significantly inhibited microglial activation and pro-inflammatory cytokine expression compared with MSCs alone. [Mol Ther Methods Clin Dev] Full Article | |
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REVIEWSMSC-extracellular vesicles (EVs) provide a novel, very promising therapeutic agent, which have already been successfully applied to humans. However, the MSC-EV production process has not been standardized, yet. Indeed, a collection of different protocols has been used for the MSC-EV production, characterization and application. By focusing on kidney, heart, liver and brain injuries, the authors reviewed the major outcomes of published MSC-EV in vivo studies. [Int J Mol Sci] Full Article Visit our reviews page to see a complete list of reviews in the mesenchymal cell research field. | |
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INDUSTRY NEWSSanBio Receives $20 Million Grant from CIRM for Stroke Clinical Trial SanBio, Inc. announced it has been awarded a $20 million grant from the California Institute of Regenerative Medicine (CIRM) in support of its Phase IIb clinical trial for the treatment of chronic stroke, jointly sponsored with Sunovion Pharmaceuticals Inc. [SanBio, Inc.] Press Release A groundbreaking randomized clinical trial (RCT) evaluating the use of a patient’s own stem cells to regenerate knee cartilage is underway. The study is the first multicenter Phase II United States FDA observed RCT of its kind. [Andrews Institute for Orthopedics & Sports Medicine] Press Release The physicians and researchers at the Rizzoli Institute are conducting the first prospective, randomized and controlled study comparing the safety and performance of a single intra-articular injection of micro-fragmented adipose tissue versus platelet rich plasma in patients with knee osteoarthritis. [Lipogems (PR Newswire Association LLC.)] Press Release | |
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POLICY NEWSTrump Administration Chooses Georgia Physician to Lead US Public-Health Agency Obstetrician and gynecologist Brenda Fitzgerald will direct the US Centers for Disease Control and Prevention, Secretary of Health and Human Services Tom Price announced. [Nature News] Editorial French Scientist Fined for Failure to Disclose Industry Ties In an unprecedented court case in Paris, an eminent French lung specialist has been fined €50,000 and given a six-month suspended jail sentence because he didn’t disclose his ties to the oil industry during a Senate air-pollution inquiry. [Nature News] Editorial White House’s Dwindling Science Office Leaves Major Research Programs in Limbo US President Donald Trump has pledged to shrink the federal government, and he seems to be starting with science. Nearly six months after taking office, Trump has not chosen a science adviser, and the White House’s Office of Science and Technology Policy has dwindled from around 130 staff members under former president Barack Obama to 35. [Nature News] Editorial
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EVENTSNEW World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESNEW Postdoctoral Fellow – Regenerative Medicine (Loma Linda University) Postdoctoral Fellow – Mesenchymal Stem Cell Research (University of Turin) Postdoctoral Fellow – Sarcoma Development (University of Turin) PhD Student Position – Skeletal Cell Biology (KU Leuven) Postdoctoral Position – Metabolism (University of North Carolina, Chapel Hill) Postdoctoral Position – Osteoarthritis (Deutsches Rheuma-Forschungszentrum) Postdoctoral Researcher – Vascular-Stromal Regulation (Weill Cornell Medical College) Postdoctoral Position – Cardiopulmonary Molecular Biology (Hannover Medical School) PhD Position – Stem Cells and Tissue Engineering (Queensland University of Technology) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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Home Mesenchymal Cell News Volume 9.27 | Jul 11 2017