Mesenchymal Cell News 9.36 September 12, 2017 | |
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TOP STORYHuman bone marrow mesenchymal stem cells with reduced SIRT7 activity were developed to evaluate the effect of SIRT7 on osteogenesis. SIRT7 knockdown significantly enhanced osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. [Cell Death Dis] Full Article | |
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PUBLICATIONS(Ranked by impact factor of the journal)IN VITROScientists developed a bioreactor that was used to culture mesenchymal stem cells harboring a strain-responsive AP-1 luciferase reporter construct, on porous scaffolds. Controlled perfusion and deformation of the three-dimensional scaffold facilitated the alteration of the expression of osteogenic markers, luciferase activity, and calcification. [J Tissue Eng Regen Med] Abstract To determine whether 5-MTP protects against stress-induced mesenchymal stem cell (MSC) senescence, researchers incubated bone marrow-derived MSC in high-glucose medium or regular medium for two weeks followed by addition of 5-MTP or vehicle for 48 hours. Since mechanistic target of rapamycin (mTOR) is involved in cellular senescence, they determined whether 5-MTP influences mTOR expression. [Sci Rep] Full Article The Role of Cellular Proliferation in Adipogenic Differentiation of Human Mesenchymal Stem Cells Using adipose tissue derived human mesenchymal stem cells as an in vitro cell model for adipogenic study, a group of cell cycle regulators including Cdk1 and CCND1 were found to be down-regulated as early as 24 hours post adipogenic initiation and consistently, cell proliferation activity was restricted to the first 48 hours of adipogenic induction. [Stem Cells Dev] Abstract Gingival mesenchymal stem cells, isolated from healthy donors, were expanded in vitro, by culturing them adherent in plastic dishes (CTR-GMSCs) or on a poly-(lactic acid) scaffold (SC-GMSCS). In order to evaluate the survival and the neurogenic differentiation potential the authors performed a comparative transcriptomic analysis between CTR-GMSCs and SC-GMSCs by next generation sequencing. [J Biomed Mater Res A] Abstract Stimulation of 3D Osteogenesis by Mesenchymal Stem Cells Using a Nanovibrational Bioreactor Researchers showed that vibrations of nanoscale amplitude provided by a newly developed bioreactor can differentiate a potential autologous cell source, mesenchymal stem cells, into mineralized tissue in 3D. They demonstrated that nanoscale mechanotransduction can stimulate osteogenesis independently of other environmental factors, such as matrix rigidity. [Nat Biomed Eng] Abstract | Press Release IN VIVOInvestigators uncovered the ability of a recently identified skin resident immunomodulatory dermal MSC marked by ABCB5 to reduce recessive dystrophic epidermolysis bullosa (RDEB) pathology and markedly extend the lifespan of RDEB NOD/SCID IL2rγcnull mice via reduced skin infiltration of inflammatory myeloid derivatives. [Lab Invest] Abstract Scientists characterized MSCs and IFNγ primed MSCs prepared with our protocol, by karyotype, in vitro potential for malignant transformation, biodistribution, effect on engraftment of transplanted hematopoietic cells, and in vivo toxicity in immune deficient mice including a complete post-mortem examination. [Stem Cells Transl Med] Full Article Researchers studied the influence of different cell densities on the behavior of erythropoietin-secreting MSCs immobilized in alginate microcapsules for their use as drug delivery systems. [Int J Pharm] Full Article | Graphical Abstract Synthetic Poly(vinylalcohol)-Based Membranes for Cartilage Surgery and Repair The authors developed poly(vinylalcohol)-based membranes to be used for mesenchymal stem cell implantation in cartilage repair procedures, having controllable physicochemical properties such as porosity, mechanical strength and permeability, and a unique self-sealing property. Although low retention of the membrane was observed in vivo in a rabbit knee model, results suggest that the membrane was able to facilitate mesenchymal stem cell implantation and differentiation to chondrocytes. [Biotechnol J] Abstract Since the bioreactor-based expansion on 3D ceramic scaffolds encompasses multiple aspects that are inherently different from expansion on 2D polystyrene, scientists aimed to decouple the effects of specific parameters among these two model systems. They assessed the effects of the: 3D scaffold vs 2D surface, ceramic vs polystyrene materials, and bone marrow-derived mesenchymal stromal cells niche established within the ceramic pores during in vitro culture, on subsequent in vivo bone formation. [Biotechnol J] Abstract | |
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REVIEWSCritical View on Mesenchymal Stromal Cells in Regenerative Medicine Researchers critically discuss clinical mesenchymal stem cell applications. They conclude that adherence to rigorous methodological rules is necessary to prevent the procedures which can be dangerous for patients and scientific research on the medical use of stem cells. [Antioxid Redox Signal] Abstract The Advancement of Biomaterials in Regulating Stem Cell Fate The authors focus on the advances of natural and synthetic biomaterials in regulating stem cell fate decisions. They also briefly discuss how biological and biophysical properties of biomaterials including wettability, chemical functionality, biodegradability and stiffness play their roles. [Stem Cell Rev] Abstract Visit our reviews page to see a complete list of reviews in the mesenchymal cell research field. | |
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INDUSTRY NEWSBone Marrow Models to Study Blood and Musculoskeletal Disorders For her research on the development of hematological and musculoskeletal disorders, Dr. Cornelia Lee-Thedieck, scientist at Karlsruhe Institute of Technology, is awarded an European Research Council (ERC) Starting Grant: The ERC decided to fund her project “bloodANDbone” with EUR 1.5 million for five years. [Karlsruhe Institute of Technology] Press Release Longeveron Initiates Phase IIb Stem Cell Therapy Trial to Treat Aging Frailty Longeveron LLC announced that it treated its first patient in the company’s Phase IIb clinical trial evaluating the safety and efficacy of longeveron human allogeneic mesenchymal stem cells in patients with Aging Frailty Syndrome. [Longeveron LLC] Press Release BioCardia®, Inc. announced the issuance of United States Patent No. 9,301,975 relating to a method of producing mesenchymal stem cells from bone marrow cells in a culture medium sourced from the same donor as the cell population being cultured. [BioCardia®, Inc.] Press Release | |
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POLICY NEWSCongress Rejects Trump Proposals to Cut Health Research Funds Congress has not only rejected the president’s National Institutes of Health proposal; lawmakers from both parties have joined forces to increase spending on biomedical research — and have bragged about it. [The New York Times] Editorial South Korean Researchers Lobby Government to Lift Human-Embryo Restrictions More than a decade after a fraud scandal in stem-cell science rocked South Korea, scientists in the field are ramping up pressure on the government to relax the country’s strict regulations on human-embryo research — which many researchers label a ban. The nation’s bioethics committee held a public forum with the Ministry of Health and Welfare in Seoul, inviting 11 researchers and scholars to discuss possible changes to the country’s bioethics policies on research. [Nature News] Editorial Pall Hangs over U.S.-Iran Science Ties Rising tensions between the U.S. and Iranian governments have frozen most scientific contacts between the two nations, experts reported at a forum. [ScienceInsider] Editorial
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EVENTSNEW World Advanced Therapies and Regenerative Medicine Congress 2018 Visit our events page to see a complete list of events in the community.
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JOB OPPORTUNITIESFaculty Position – Stem Cell Biology (University of Texas Southwestern Medical Center) Researcher – Skeletal Tissue Engineering (KU Leuven) Faculty Position – Regenerative Biology (Northwestern University) Professor – Bioengineering (University of Pittsburgh) Assistant Professor – Human Anatomy and Cell Science (University Of Manitoba) PhD Position – Stem Cells and Tissue Engineering (Queensland University of Technology) Recruit Top Talent: Reach potential candidates by posting your organization’s career opportunities on the Connexon Creative Job Board at no cost.
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