Cultured Networks of Excitatory Projection Neurons and Inhibitory Interneurons for Studying Human Cortical Neurotoxicity Scientists developed a human cortical neuron culture system from human embryonic stem cells or human inducible pluripotent stem cells that generated both excitatory and inhibitory neuronal networks resembling the composition of the human cortex. [Sci Transl Med] Abstract Cranial Grafting of Stem Cell-Derived Microvesicles Improves Cognition and Reduces Neuropathology in the Irradiated Brain Investigators provide the first evidence that cranial grafting of microvesicles secreted from human neural stem cell affords similar neuroprotective phenotypes after head-only irradiation. [Proc Natl Acad Sci USA] Abstract | Full Article | Press Release In Vitro System Using Human Neurons Demonstrates that Varicella-Zoster Vaccine Virus Is Impaired for Reactivation, but not Latency The authors found that axonal infection of neurons derived from hESCs in a microfluidic device with cell-free parental Oka varicella-zoster virus resulted in latent infection with inability to detect several viral mRNAs by reverse transcriptase-quantitative PCR, no production of infectious virus, and maintenance of the viral DNA genome in endless configuration, consistent with an episome configuration. [Proc Natl Acad Sci USA] Abstract Formation of Neuronal Circuits by Interactions between Neuronal Populations Derived from Different Origins in the Drosophila Visual Center Researchers demonstrated that cell-cell interactions play an important role in establishing the arrangement of neurons of different origins in the Drosophila visual center. Specific types of neurons born outside the medulla primordium migrate tangentially into the developing medulla cortex. [Cell Rep] Full Article | Graphical Abstract Mechanosensory Organ Regeneration in Zebrafish Depends on a Population of Multipotent Progenitor Cells Kept Latent by Schwann Cells The authors used electroablation in transgenic larvae expressing fluorescent proteins in posterior lateral line (PLL) components to completely ablate single neuromasts in larvae and adult fish. This injury resulted in discontinuity of the interneuromastic cells, Schwann cells, and the PLL nerve. [BMC Biol] Full Article Prox1 Is Required for Oligodendrocyte Cell Identity in Adult Neural Stem Cells of the Subventricular Zone Scientists showed that within the subventricular zone (SVZ) Prox1 induces differentiation into oligodendrocytes. They found that loss of Prox1 expression in vivo reduced cell migration into the corpus callosum, where the few Prox1 deficient SVZ- derived remaining cells fail to differentiate into oligodendrocytes. [Stem Cells] Abstract Cell-Permeable p38 MAP Kinase Promotes Migration of Adult Neural Stem/Progenitor Cells Researchers showed that p38 MAP kinase (p38) is expressed in doublecortin-positive adult neural stem/progenitor cells (NPCs). Experiments using the p38 inhibitor SB203580 revealed that endogenous p38 participates in NPC migration. [Sci Rep] Full Article Neural Ablation of the PARK10 Candidate Plpp3 Leads to Dopaminergic Transmission Deficits without Neurodegeneration Investigators showed that phospholipid phosphatase 3 (PLPP3) is enriched in astroglial cells of the adult murine ventral midbrain. Conditional inactivation of Plpp3 using a Nestin::Cre driver resulted in reduced mesencephalic levels of sphingosine-1-phosphate receptor 1, a well-known mediator of pro-survival responses. [Sci Rep] Full Article KCa3.1 Inhibition Switches the Phenotype of Glioma-Infiltrating Microglia/Macrophages Researchers determined whether Ca2+-activated K+ channel (KCa3.1) inhibition could prevent the acquisition of a pro-tumor phenotype by microglia/macrophage (M/MΦ) cells, thus contributing to reduce glioma development. They studied microglia cultured in glioma-conditioned medium or treated with IL-4, as well as M/MΦ cells acutely isolated from glioma-bearing mice and from human glioma biopsies. [Cell Death Dis] Full Article Rett Syndrome Mutant Neural Cells Lacks MeCP2 Immunoreactive Bands The purpose of this study was to advance understanding of multiple MeCP2 immunoreactive bands in control neural cells and p.T158M MeCP2e1 mutant cells. Scientists generated stable wild-type and p.T158M MeCP2e1-RFP mutant expressing cells. [PLoS One] Full Article Shift of MicroRNA Profile upon Orthotopic Xenografting of Glioblastoma Spheroid Cultures Scientists aimed to identify over-expressed tumor cells with stem-like properties (TSC)-related microRNAs potentially amenable for therapeutic targeting. They used non-differentiated glioblastoma spheroid cultures containing TSCs and compared these to xenografts using a NanoString nCounter platform. [J Neurooncol] Abstract |