Researchers showed that the isolated brain-derived neurotrophic factor (BDNF) prodomain is detected in the hippocampus and that it can be secreted from neurons in an activity-dependent manner. [Nat Commun] Abstract Membrane Biophysics Define Neuron and Astrocyte Progenitors in the Neural Lineage
Researchers hypothesized that inherent electrophysiological properties are sufficient to define neurogenic progenitors (NPs) and astrogenic progenitors (APs) and test this by determining whether isolation of cells solely by these properties specifically separates NPs and APs. They found NPs and APs are enriched in distinct fractions after separation by electrophysiological properties using dielectrophoresis. [Stem Cells] Abstract
Interplay between Human Microglia and Neural Stem/Progenitor Cells in an Allogeneic Co-Culture Model
Scientists focused their studies on the interaction of human neural stem/progenitor cells (NPCs) and microglia utilizing a co-culture model. In co-cultures, both NPCs and microglia showed increased survival and proliferation compared with mono-cultures. [J Cell Mol Med] Abstract | Full Article
Cyclic AMP Promotes Axon Regeneration, Lesion Repair and Neuronal Survival in Lampreys after Spinal Cord Injury
Scientists took advantage of the large, identified reticulospinal neurons in lamprey, a vertebrate that exhibits robust axon regeneration after a complete spinal cord transection. Application of a cyclic adenosine monophosphate (cAMP) analog, db-cAMP, at the time of spinal cord transection increased the number of axons that regenerated across the lesion site. [Exp Neurol] Abstract
Short-Term Episodes of Hypoxia Induce Posthypoxic Hyperexcitability and Selective Death of GABAergic Hippocampal Neurons
Researchers compared the effect of short-term hypoxia on the amplitude of the Ca2+ response to NMDA and the Ca2+ transients generation in two populations of neurons – inhibitory GABAergic and excitatory glutamatergic. [Exp Neurol] Abstract
Molecular Features of Neural Stem Cells Enable their Enrichment Using Pharmacological Inhibitors of Survival-Promoting Kinases
Researchers demonstrated that postnatal rat neural precursors express low levels of pro-apoptotic molecules and resist PI3K and ERK1/2 inhibition as compared to late oligodendrocyte progenitors. [J Neurochem] Abstract
Differentiation of Reprogrammed Human Adipose Mesenchymal Stem Cells toward Neural Cells with Defined Transcription Factors
Researchers report a reprogramming methodology with which human adipose stem cells can be differentiated into neural cells. [Biochem Biophys Res Commun] Abstract
Migration of Glial Cells Differentiated from Neurosphere-Forming Neural Stem/Progenitor Cells Depends on the Stiffness of the Chemically Cross-Linked Collagen Gel Substrate
Substrate stiffness affects cell migration and spreading. Researchers revealed that the stiffness of the cell-adhesive substrate affected the migration pattern of neural cells. [Neurosci Lett] Abstract
Interactive Effects of Cell Therapy and Rehabilitation Realize the Full Potential of Neurogenesis in Brain Injury Model
Researchers report the neural stem/progenitor cells transplantation into a brain injury mouse model followed by treadmill exercise training. Transplanted cells at the brain injury site were observed and differentiated into neurons and astrocytes. [Neurosci Lett] Abstract
Imaging-Based Chemical Screening Reveals Activity-Dependent Neural Differentiation of Pluripotent Stem Cells
Investigators report a high throughput imaging-based screen, which uncovers that selamectin, an anti-helminthic therapeutic compound with reported activity on invertebrate glutamate-gated chloride channels, promotes neural differentiation of pluripotent stem cells. [eLife] Full Article