Dynamin-Related Protein 1 Is Required for Normal Mitochondrial Bioenergetic and Synaptic Function in CA1 Hippocampal Neurons Using ATP FRET sensors, scientists found that cultured neurons lacking dynamin-related protein 1 could not maintain normal levels of mitochondrial-derived ATP when energy consumption was increased by neural activity. These deficits occurred specifically at the nerve terminal, but not the cell body, and were sufficient to impair synaptic vesicle cycling. [Cell Death Dis] Full Article The Regulation of p53 Upregulated Modulator of Apoptosis (Puma) by JNK/c-Jun Pathway in β-Amyloid Induced Neuron Death Researchers demonstrated that the JNK/c-Jun pathway is activated, in cultures of cortical neurons following treatment with oligomeric β-amyloid (Aβ) and in Alzheimer’s disease transgenic mice, and that inhibition of this pathway by selective inhibitor blocks induction of Puma by Aβ. [J Neurochem] Abstract Collybistin Binds and Inhibits mTORC1 Signaling: A Potential Novel Mechanism Contributing to Intellectual Disability and Autism By using induced pluripotent stem cell-derived neural progenitor cells from a male patient with a deletion of entire collybistin (CB) gene and from control individuals, as well as a heterologous expression system, researchers describe that CB physically interacted with mammalian target of rapamycin (mTOR) and inhibited mTOR complex 1 (mTORC1) signaling pathway and protein synthesis. [Eur J Hum Genet] Abstract Differentiation of Human Neural Stem Cells into Motor Neurons Stimulates Mitochondrial Biogenesis and Decreases Glycolytic Flux To test if mitobiogenesis is increased during motor neuron differentiation, scientists differentiated human embryonic stem cell and induced pluripotent stem cell-derived human neural stem cells into motor neurons. [Stem Cells Dev] Abstract Toward a Broader View of Ube3a in a Mouse Model of Angelman Syndrome: Expression in Brain, Spinal Cord, Sciatic Nerve and Glial Cells Findings regarding Ube3a expression in non-neuronal cells of the brain have varied. Utilizing enriched primary cultures of oligodendrocytes and astrocytes, the authors showed that Ube3a is expressed, but not imprinted in these cell types. [PLoS One] Full Article Periostin Promotes Neural Stem Cell Proliferation and Differentiation following Hypoxic-Ischemic Injury Scientists investigated the role of periostin (POSTN) in neural stem cells (NSCs) following hypoxia-ischemia. They found that POSTN mRNA levels significantly increased in differentiating NSCs. [PLoS One] Full Article Diallyl Trisufide Protects against Oxygen Glucose Deprivation-Induced Apoptosis by Scavenging Free Radicals via the PI3K/Akt -Mediated Nrf2/HO-1 Signaling Pathway in B35 Neural Cells Scientists used oxygen glucose deprivation (OGD) model to investigate the neuroprotective effects of diallyl trisulfide and the mechanisms related to these effects. B35 neural cells exposed to OGD caused a decrease in cell viability and increased in the percentage of apoptotic cells and the level of intracellular cleaved caspase-3, all of which were markedly attenuated by DATS. [Brain Res] Abstract Capillarisin Suppresses Lipopolysaccharide-Induced Inflammatory Mediators in BV2 Microglial Cells by Suppressing TLR4-Mediated NF-κB and MAPKs Signaling Pathway The authors investigated the anti-inflammatory effects and molecular mechanisms of capillarisin on LPS-stimulated BV2 microglial cells. The effects of capillarisin on inflammatory mediators TNF-α, IL-6, IL-1β, NO and PGE2 were detected. [Neurochem Res] Abstract Microglia in Glia-Neuron Co-Cultures Exhibit Robust Phagocytic Activity without Concomitant Inflammation or Cytotoxicity A simple method to co-culture granule neurons and glia from a single brain region is described, and microglia activation profiles were assessed in response to naturally occurring neuronal apoptosis, excitotoxin-induced neuronal death, and lipopolysaccharide addition. [Cell Mol Neurobiol] Abstract |