Association of Astrocytes with Neurons and Astrocytes Derived from Distinct Progenitor Domains in the Subpallium Whether astrocytes can only interact with neurons from common progenitor domains in the brain remains unknown. Scientists focused on subpallium-derived cells, because the subpallium generates neurons that show a diverse mode of migration. They tracked their fate by in utero electroporation of plasmids that allow for chromosomal integration of transgenes or of a Cre recombinase expression vector to reporter mice. [Sci Rep] Full Article A Standardized and Reproducible Protocol for Serum-Free Monolayer Culturing of Primary Pediatric Brain Tumors to Be Utilized for Therapeutic Assays Researchers defined a simple, non-expensive and reproducible serum-free cell culture protocol for establishment and propagation of primary pediatric brain tumor cultures as adherent monolayers. [Sci Rep] Full Article Engrafted Neural Stem/Progenitor Cells Promote Functional Recovery through Synapse Reorganization with Spared Host Neurons after Spinal Cord Injury Scientists showed that neural stem/progenitor cell (NSPC) transplantation promotes functional recovery after mild and moderate spinal cord injury (SCI), but not after severe SCI. In severe SCI mice, there were few remaining host neurons within the range of NSPC engraftment; thus, they examined whether the co-distribution of transplant and host is a contributory factor for functional improvement. [Stem Cell Reports] Full Article Interferon Gamma Induces Protective Non-Canonical Signaling Pathways in Primary Neurons Investigators showed that exogenous interferon γ (IFNγ) has no deleterious impact on neuronal viability, and staurosporine-induced apoptosis in neurons is significantly blunted by the addition of IFNγ, suggesting that IFNγ confers a pro-survival signal in neurons. [J Neurochem] Abstract Neuronal Regulation of Neuroprotective Microglial Apolipoprotein E Secretion in Rat In Vitro Models of Brain Pathophysiology Using rat primary neural cell cultures, scientists showed that microglia release apolipoprotein E (ApoE) through a Golgi-mediated secretion pathway and that ApoE progressively accumulates in neuroprotective microglia-conditioned medium. This constitutive ApoE release is negatively affected by microglial activation both with lipopolysaccharide and with ATP. [J Neuropathol Exp Neurol] Abstract Endoscopic Delivery of Enteric Neural Stem Cells to Treat Hirschsprung Disease Enteric neural stem cells for transplantation were cultured from dissociated gut of postnatal donor mice. Twelve recipient mice, including Ednrb−/− mice with distal colonic aganglionosis, underwent colonoscopic injection of enteric neural stem cells under direct vision using a 30-gauge Hamilton needle passed through a rigid cystoureteroscope. [Neurogastroenterol Motil] Abstract Chronaxie Measurements in Patterned Neuronal Cultures from Rat Hippocampus The authors used patterned quasi one-dimensional neuronal cultures from rat hippocampus, exploiting the alignment of axons along the linear patterned culture to separate the contribution of dendrites to the excitation of the neuron from that of axons. [PLoS One] Full Article In Vitro Screening of Clinical Drugs Identifies Sensitizers of Oncolytic Viral Therapy in Glioblastoma Stem-Like Cells Investigators addressed the feasibility of clinically applicable drugs to enhance the oncolytic potential of the oncolytic virus Delta24-RGD in glioblastoma. In total, 446 drugs were screened for their viral sensitizing properties in glioblastoma stem-like cells in vitro. [Gene Ther] Abstract The Neuritogenic and Synaptogenic Effects of the Ethanolic Extract of Radix Puerariae in Cultured Rat Hippocampal Neurons The authors investigated the neurotrophic effects of these ethnomedicines on the development of central nervous system neurons and the molecular bases of these activities. [J Ethnopharmacol] Abstract |