Retinoic Acid Induces Ubiquitination-Resistant RIP140/LSD1 Complex to Fine-Tune Pax6 Gene in Neuronal Differentiation Investigators reveal a new retinoic acid (RA)-induced gene repressive mechanism that modulates the abundance, enzyme quality and recruitment of histone modifier lysine-specific demethylase 1 (LSD1) to neuronal regulator Pax6, which provides a homeostatic control for RA induction of neuronal differentiation. [Stem Cells] Abstract Proton Beam Radiation Induces DNA Damage and Cell Apoptosis in Glioma Stem Cells through Reactive Oxygen Species By utilizing patient-derived glioma stem cells (GSCs), researchers showed that proton radiation generates greater cytotoxicity in GSCs than x-ray photon radiation. Compared with photon radiation, proton beam irradiation induces more single and double strand DNA breaks, less H2AX phosphorylation, increased Chk2 phosphorylation, and reduced cell cycle recovery from G2 arrest, leading to caspase-3 activation, PARP cleavage, and cell apoptosis. [Sci Rep] Full Article CEND1 and NEUROGENIN2 Reprogram Mouse Astrocytes and Embryonic Fibroblasts to Induced Neural Precursors and Differentiated Neurons Investigators explored the effect of CEND1 and NEUROG2 on reprogramming of mouse cortical astrocytes and embryonic fibroblasts. Forced expression of CEND1, NEUROG2, or both resulted in acquisition of induced neuronal cells expressing subtype-specific markers, while long-term live-cell imaging highlighted the existence of two different modes of neuronal trans-differentiation. [Stem Cell Reports] Full Article Effect of Cholesterol Reduction on Receptor Signaling in Neurons Researchers explored the effects of the decrease in cholesterol on neuronal cell function using GT1-7 hypothalamic cells subjected to cholesterol depletion in vitro using three independent methods: exposure to methyl-beta-cyclodextrin, treatment with the HMG-CoA reductase inhibitor simvastatin and shRNA-mediated knockdown of SREBP2. [J Biol Chem] Abstract | Full Article Hippocampal PPARδ Overexpression or Activation Represses Stress-Induced Depressive Behaviors and Enhances Neurogenesis In vivo or in vitro studies showed that both overexpression and activation of peroxisome proliferator-activated receptor δ (PPARδ) enhanced proliferation or differentiation of neural stem cells in the hippocampi of mice. [Int J Neuropsychopharmacol] Full Article Transdifferentiation-Induced Neural Stem Cells Promote Recovery of Middle Cerebral Artery Stroke Rats Scientists produced transdifferentiated induced neural stem cells (iNSCs) by conditional overexpressing Oct4, Sox2, Klf4, c-Myc in mouse embryonic fibroblasts. They expanded readily in vitro and expressed NSC mRNA profile and protein markers. These iNSCs differentiated into mature astrocytes, neurons and oligodendrocytes in vitro. [PLoS One] Full Article Phenytoin Enhances the Phosphorylation of EGFR and FGFR in the Subventricular Zone and Promotes the Proliferation of Neural Precursor Cells and Oligodendrocyte Differentiation Scientists determined the cell lineage and cell fate of ventricular-subventricular zone neural progenitors expanded by phenytoin, as well as the effects of this drug on epidermal growth factor receptor (EGFR)/fibroblast growth factor (FGFR) phosphorylation. [Eur J Neurosci] Abstract Morphine Preconditioning Downregulates MicroRNA-134 Expression against Oxygen-Glucose Deprivation Injuries in Cultured Neurons of Mice Morphine preconditioning induces neuroprotection in neurons, but it remains uncertain whether microRNA-134 is involved in morphine preconditioning against oxygen-glucose deprivation-induced injuries in primary cortical neurons of mice. The authors examined this issue. [J Neurosurg Anesthesiol] Abstract Involvement of Caspase-3/PTEN Signaling Pathway in Isoflurane-Induced Decrease of Self-Renewal Capacity of Hippocampal Neural Precursor Cells Investigators used in vitro neural precursor cells (NPCs) cultures to test whether caspase-3 and its downstream signaling effectors were involved in isoflurane-induced impairment of the self-renewal capacity of hippocampal NPCs. [Brain Res] Abstract Tanshinone IIA, a Multiple Target Neuroprotectant, Promotes Caveolae-Dependent Neuronal Differentiation Tanshinone IIA, a multiple target neuroprotectant, was demonstrated to promote dose-dependent neuronal differentiation in three cell models of immortalized C17.2 neuronal stem cells, rat embryonic cortical neural stem cells and rat PC12 pheochromocytoma cells. [Eur J Pharmacol] Abstract |