Organoid News Volume 1.15 | Oct 22 2020

May 12, 2022

ON 1.15 | Oct 22 2020

Vol. 1.15 – 22 October, 2020

TOP STORY

Single-Cell
RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-Like Cells Derived from Human Pluripotent Stem Cells

Researchers purified β-like cells for spontaneous formation of islet-like organoids against CD9, and found significantly more NKX6.1⁺MAFA⁺C-PEPTIDE⁺ β-like cells in the CD9^– than in the CD9⁺ population.
[Cell Stem Cell]

Full Article Graphical Abstract

Survey Report: Where Is Pluripotent Stem Cell Research Now?

PUBLICATIONSRanked by the impact factor of the journal

Automated
Microfluidic Platform for Dynamic and Combinatorial Drug Screening of Tumor Organoids

Scientists present an automated, high-throughput, microfluidic 3D organoid culture and analysis system to facilitate preclinical research and personalized therapies. They validated the system by performing individual, combinatorial, and sequential drug screens on human-derived pancreatic tumor organoids.
[Nature Communications]

Full Article

Single-Cell
Derived Tumor Organoids Display Diversity in HLA Class I Peptide Presentation

The authors investigated the variability in HLA class I peptide presentation between different clonal cells of the same colorectal cancer patient, using an organoid system.
[Nature Communications]

Full Article

Cell-Cycle-Dependent
ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo

Endogenous tagging of Nanog in ESCs revealed that ERK inhibition promoted enhanced stabilization of NANOG protein after mitosis. Scientists showed that cell cycle, signaling, and differentiation are coordinated during preimplantation development.
[Developmental Cell]

Full Article Graphical Abstract

Complete
Inhibition of ABCB1 and ABCG2 at the Blood–Brain Barrier by Co-Infusion of Erlotinib and Tariquidar to Improve Brain Delivery of the Model ABCB1/ABCG2 Substrate [¹¹C]erlotinib

Scientists examined the feasibility of simultaneous ABCB1/ABCG2 inhibition with i.v. co-infusion of erlotinib and tariquidar by studying brain distribution of the model ABCB1/ABCG2 substrate erlotinib in mice and rhesus macaques with PET. Tolerability of the erlotinib/tariquidar combination was assessed in human embryonic stem cell-derived cerebral organoids.
[Journal of Cerebral Blood Flow and Metabolism]

Abstract

Elimination
of Nf-kB Signaling in Vimentin+ Stromal Cells Attenuates Tumorigenesis in a Mouse Model of Barrett Esophagus

To analyze the effect of NF-kB signaling in myofibroblasts (MFs), researchers crossed L2-IL-1B mice to tamoxifen inducible Vim-Cre mice and floxed RelA mice to specifically eliminate NF-kB signaling in MF. The interaction of epithelial cells and stromal cells was further analyzed in mouse Barrett Esophagus organoids and patient-derived human organoids.
[Carcinogenesis]

Abstract

Oncogenic
KRAS-Expressing Organoids with Biliary Epithelial Stem Cell Properties Give Rise to Biliary Tract Cancer in Mice

Researchers isolated epithelial cell adhesion molecule-positive biliary epithelial cells from the mouse intrahepatic bile duct, gallbladder, and extrahepatic bile duct and established organoids derived from these cells.
[Cancer Science]

Abstract

Evaluation
of the RAS Signaling Network in Response to MEK Inhibition Using Organoids Derived from a Familial Adenomatous Polyposis Patient

Investigators used patient-derived organoids derived from a familial adenomatous polyposis patient to analyze the response to chemotherapeutic agents targeting EGFR, BRAF and MEK.
[Scientific Reports]

Full Article

Anti-GD2-IRDye800CW
as a Targeted Probe for Fluorescence-Guided Surgery in Neuroblastoma

Scientists implemented a xenograft model of patient-derived neuroblastoma organoids with differential GD2 expression and showed that even low GD2 expressing tumors still provided an adequate real-time fluorescence signal.
[Scientific Reports]

Full Article

Breast
Cancer Organoid Model Allowed to Reveal Potentially Beneficial Combinations of 3,3′-Diindolylmethane and Chemotherapy Drugs

A tumor organoid culture from metastatic breast cancer was established. Morphological, IHC, FACS and cytogenetics analysis confirmed that organoids share morphologic features with the primary tumor and possess unique cytogenetic characteristics.
[Biochimie]

Abstract

Virtual Conference Exhibition: Organoids

REVIEWS

Organoids to Model Liver Disease

Organoid models offer new perspectives in personalized medicine and drug discovery. The authors discuss the current liver organoid models for the study of liver disease.
[JHEP Reports]

Full Article

Modeling Human Neuronal Migration Deficits in 3D

Scientists provide a short introduction on human and mouse neuronal migration processes, and highlight human brain organoid models of neuronal migration diseases.
[Current Opinion in Neurobiology]

Full Article

Potential
Ethical Problems with Human Cerebral Organoids: Consciousness and Moral Status of Future Brains in a Dish

The author discusses the conditions under which human cerebral organoids could require the acknowledgment of their own moral status.
[Brain Research]

Abstract

INDUSTRY AND POLICY NEWS

Lineage
Cell Therapeutics and Cancer Research UK Announce Encouraging Preliminary Phase I Study Results with VAC2 for the Treatment of Non-Small Cell Lung Cancer

Lineage Cell Therapeutics, Inc. and Cancer Research UK have announced encouraging preliminary results from an ongoing Phase I clinical study of VAC2 in non-small cell lung cancer (NSCLC). Produced from a pluripotent cell technology using a directed differentiation method, VAC2 has demonstrated remarkably potent induction of immune responses in all patients dosed to date.
[Lineage Cell Therapeutics, Inc.]

Press Release

FEATURED EVENT

Single-Cell
RNA-Seq Reveals that CD9 Is a Negative Marker of Glucose-Responsive Pancreatic β-Like Cells Derived from Human Pluripotent Stem Cells

Automated
Microfluidic Platform for Dynamic and Combinatorial Drug Screening of Tumor Organoids

Single-Cell
Derived Tumor Organoids Display Diversity in HLA Class I Peptide Presentation

Cell-Cycle-Dependent
ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo

Complete
Inhibition of ABCB1 and ABCG2 at the Blood–Brain Barrier by Co-Infusion of Erlotinib and Tariquidar to Improve Brain Delivery of the Model ABCB1/ABCG2 Substrate [¹¹C]erlotinib

Elimination
of Nf-kB Signaling in Vimentin+ Stromal Cells Attenuates Tumorigenesis in a Mouse Model of Barrett Esophagus

Oncogenic
KRAS-Expressing Organoids with Biliary Epithelial Stem Cell Properties Give Rise to Biliary Tract Cancer in Mice

Evaluation
of the RAS Signaling Network in Response to MEK Inhibition Using Organoids Derived from a Familial Adenomatous Polyposis Patient

Anti-GD2-IRDye800CW
as a Targeted Probe for Fluorescence-Guided Surgery in Neuroblastoma

Breast
Cancer Organoid Model Allowed to Reveal Potentially Beneficial Combinations of 3,3′-Diindolylmethane and Chemotherapy Drugs

Organoids to Model Liver Disease

Modeling Human Neuronal Migration Deficits in 3D

Potential
Ethical Problems with Human Cerebral Organoids: Consciousness and Moral Status of Future Brains in a Dish

Lineage
Cell Therapeutics and Cancer Research UK Announce Encouraging Preliminary Phase I Study Results with VAC2 for the Treatment of Non-Small Cell Lung Cancer

Keystone
Symposia: Emerging Cell Therapies: Realizing the Vision of NextGen Cell Therapeutics

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Research
Positions – Organs on a Chip

Research Associate – Neuroscience and Stem Cell Biology

Faculty Position – Stem Cells and Regenerative Medicine

Assistant/Associate
Professor – Stem Cells and Organoid Models for Human Disease

Postdoctoral position – Neuroscience, Stem Cell Biology, Molecular Genetics and Bioinformatics

> See All Jobs