Organoid News Volume 1.16 | Oct 29 2020

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    ON 1.16 | Oct 29 2020


    Organoid News by STEMCELL Technologies
    Vol. 1.16 – 29 October, 2020
    TOP STORY

    Identification of SARS-CoV-2 Inhibitors Using Lung and Colonic Organoids

    As SARS-CoV-2 primarily infects the respiratory tract, investigators developed a lung organoid model using human pluripotent stem cells (hPSC), and generated complementary hPSC-derived colonic organoids to explore the response of colonic cells to SARS-CoV-2 infection.
    [Nature]

    Full ArticlePress Release

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    PUBLICATIONSRanked by the impact factor of the journal

    A
    Human Tissue Screen Identifies a Regulator of ER Secretion as a Brain Size Determinant

    Scientists present CRISPR-LIneage tracing at Cellular resolution in Heterogenous Tissue, enabling parallel loss-of-function studies in human cerebral organoid tissue.
    [Science]

    AbstractPress Release

    Activated
    Microglia Cause Metabolic Disruptions in Developmental Cortical Interneurons That Persist in Interneurons from Individuals with Schizophrenia

    Researchers generated developmental cortical interneurons — which are known to be affected in schizophrenia when matured — from induced pluripotent stem cells derived from healthy controls and individuals with schizophrenia and co-cultured them with or without activated microglia.
    [Nature Neuroscience]

    Abstract

    Prime Editing for Functional Repair in Patient-Derived Disease Models

    Prime editing functionally recovered disease-causing mutations in intestinal organoids from patients with DGAT1-deficiency and liver organoids from a patient with Wilson disease
    [Nature Communications]

    Full Article

    Generation
    of a Transplantable Population of Human iPSC-Derived Retinal Ganglion Cells

    Scientists describe the generation of functional retinal ganglion cells (RGCs) from iPSCs based on innovative 3D/2D stepwise differentiation protocol. They demonstrated that targeting the cell surface marker THY1 was an effective strategy to select transplantable RGCs.
    [Frontiers in Cell and Developmental Biology]

    Full Article

    A
    Simple Metastatic Brain Cancer Model Using Human Embryonic Stem Cell-Derived Cerebral Organoids

    Investigators developed a simple 3D in vitro model to better replicate brain metastasis using human cancer cells and human embryonic stem cell‐derived cerebral organoids.
    [Faseb Journal]

    Full Article

    Identification
    of AAV Serotypes for Lung Gene Therapy in Human Embryonic Stem Cell-Derived Lung Organoids

    Researchers screened the ability of multiple adeno-associated virus serotypes to transduce lung bud organoids, a model of human lung parenchyma generated from human embryonic stem cells.
    [Stem Cell Research & Therapy]

    Full Article

    Interplay Among p21Waf1/Cip1,
    MUSASHI-1 and Krüppel-Like Factor 4 in Activation of Bmi1-CreER Reserve Intestinal Stem Cells After Gamma Radiation-Induced Injury

    Bmi1-specific Klf4 deletion resulted in decreased numbers of MSI1+ cells in regenerating crypts compared to those of control mice. Researchers showed that KLF4 bound to the Msi1 promoter and activated its expression in vitro.
    [Scientific Reports]

    Full Article

    NMR Microsystem for Label-Free Characterization of 3D Nanoliter Microtissues

    Scientists introduced a novel microsystem, which combined complementary metal-oxide semiconductor technology with 3D microfabrication, enabling nanoliter NMR as a platform tool for non-invasive spectroscopy of organoids, 3D cell cultures, and early stage embryos.
    [Scientific Reports]

    Full Article

    Scaffolds
    Obtained from Decellularized Human Extrahepatic Bile Ducts Support Organoids to Establish Functional Biliary Tissue in a Dish

    The authors demonstrated efficient decellularization of extrahepatic bile ducts (EBD) tissue. Recellularization of the EBD extracellular matrix (ECM) with the organoids of extrahepatic origin showed more profound repopulation the ductal ECM when compared to liver tissue derived organoids.
    [Biotechnology and Bioengineering]

    Abstract

    Development
    and Characterization of Rat Duodenal Organoids for ADME and Toxicology Applications

    Intestinal stem cells were isolated from adult rat duodenum and culture conditions were optimized to promote the growth, differentiation and development of 3D organoids. Investigators optimized and characterized rat duodenal organoids with light and electron microscopy, immunofluorescence and notably, global mRNA expression.
    [Toxicology]

    Abstract

    Optimal, Large-Scale Propagation of Mouse Mammary Tumor Organoids

    Using time-lapse imaging of mouse mammary tumor organoids in 3D culture, researchers observed that outgrowth potential varied non-linearly with initial organoid size.
    [Journal of Mammary Gland Biology and Neoplasia]

    Full Article

    Exogenous
    L-Arginine Increases Intestinal Stem Cell Function through CD90+ Stromal Cells Producing mTORC1-Induced Wnt2b

    Scientists utilized mice and small intestinal organoid models to clarify the role of L-arginine on epithelial differentiation of intestinal stem cells (ISCs). They showed that L-arginine increased expansion of ISCs in mice.
    [Communications Biology]

    Full Article

    Survey Report: Where Is Pluripotent Stem Cell Research Now?
    REVIEWS

    Intestinal Organoid/Enteroid-Based Models for Cryptosporidium

    Scientists discuss recent breakthroughs in the field and highlight different models for functional ex vivo organoid or enteroidderived culture systems.
    [Current Opinion in Microbiology]

    Full Article

    INDUSTRY AND POLICY NEWS

    AgeX
    Therapeutics Sublicenses Stem Cell Line ESI-053 to ImStem Biotechnology for Development of Cell Therapy Candidate IMS001 for COVID-19 and Acute Respiratory Distress Syndrome

    AgeX Therapeutics, Inc. and ImStem Biotechnology, Inc. announced that ImStem has obtained from AgeX a non-exclusive, royalty-bearing sublicense to use AgeX’s clinical-grade ESC line ESI-053 to derive ImStem’s investigational MSC product candidate IMS001 for development in COVID-19 as well as acute respiratory distress syndrome from other causes.
    [AgeX Therapeutics]

    Press Release

    FEATURED EVENT

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    May 16 – May 21, 2021
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