| Vol. 1.16 – 29 October, 2020 |
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| As SARS-CoV-2 primarily infects the respiratory tract, investigators developed a lung organoid model using human pluripotent stem cells (hPSC), and generated complementary hPSC-derived colonic organoids to explore the response of colonic cells to SARS-CoV-2 infection. [Nature] |
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| PUBLICATIONSRanked by the impact factor of the journal |
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| Scientists present CRISPR-LIneage tracing at Cellular resolution in Heterogenous Tissue, enabling parallel loss-of-function studies in human cerebral organoid tissue. [Science] |
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| Researchers generated developmental cortical interneurons — which are known to be affected in schizophrenia when matured — from induced pluripotent stem cells derived from healthy controls and individuals with schizophrenia and co-cultured them with or without activated microglia. [Nature Neuroscience] |
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| Prime editing functionally recovered disease-causing mutations in intestinal organoids from patients with DGAT1-deficiency and liver organoids from a patient with Wilson disease [Nature Communications] |
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| Scientists describe the generation of functional retinal ganglion cells (RGCs) from iPSCs based on innovative 3D/2D stepwise differentiation protocol. They demonstrated that targeting the cell surface marker THY1 was an effective strategy to select transplantable RGCs. [Frontiers in Cell and Developmental Biology] |
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| Investigators developed a simple 3D in vitro model to better replicate brain metastasis using human cancer cells and human embryonic stem cell‐derived cerebral organoids. [Faseb Journal] |
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| Researchers screened the ability of multiple adeno-associated virus serotypes to transduce lung bud organoids, a model of human lung parenchyma generated from human embryonic stem cells. [Stem Cell Research & Therapy] |
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| Bmi1-specific Klf4 deletion resulted in decreased numbers of MSI1+ cells in regenerating crypts compared to those of control mice. Researchers showed that KLF4 bound to the Msi1 promoter and activated its expression in vitro. [Scientific Reports] |
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| Scientists introduced a novel microsystem, which combined complementary metal-oxide semiconductor technology with 3D microfabrication, enabling nanoliter NMR as a platform tool for non-invasive spectroscopy of organoids, 3D cell cultures, and early stage embryos. [Scientific Reports] |
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| The authors demonstrated efficient decellularization of extrahepatic bile ducts (EBD) tissue. Recellularization of the EBD extracellular matrix (ECM) with the organoids of extrahepatic origin showed more profound repopulation the ductal ECM when compared to liver tissue derived organoids. [Biotechnology and Bioengineering] |
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| Intestinal stem cells were isolated from adult rat duodenum and culture conditions were optimized to promote the growth, differentiation and development of 3D organoids. Investigators optimized and characterized rat duodenal organoids with light and electron microscopy, immunofluorescence and notably, global mRNA expression. [Toxicology] |
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| Using time-lapse imaging of mouse mammary tumor organoids in 3D culture, researchers observed that outgrowth potential varied non-linearly with initial organoid size. [Journal of Mammary Gland Biology and Neoplasia] |
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| Scientists utilized mice and small intestinal organoid models to clarify the role of L-arginine on epithelial differentiation of intestinal stem cells (ISCs). They showed that L-arginine increased expansion of ISCs in mice. [Communications Biology] |
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| Scientists discuss recent breakthroughs in the field and highlight different models for functional ex vivo organoid or enteroidderived culture systems. [Current Opinion in Microbiology] |
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| AgeX Therapeutics, Inc. and ImStem Biotechnology, Inc. announced that ImStem has obtained from AgeX a non-exclusive, royalty-bearing sublicense to use AgeX’s clinical-grade ESC line ESI-053 to derive ImStem’s investigational MSC product candidate IMS001 for development in COVID-19 as well as acute respiratory distress syndrome from other causes. [AgeX Therapeutics] |
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| May 16 – May 21, 2021 Lucca, Italy |
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| Ncardia – Leiden, The Netherlands |
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| The University of British Columbia – Vancouver, British Columbia, Canada |
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| Baylor College of Medicine – Houston, Texas, United States |
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| Terasaki Research Institute – Los Angeles, California, United States |
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| Albert Einstein College of Medicine – New York, New York, United States |
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