Organoid News Volume 2.04 | Feb 4 2021

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    ON 2.04 | Feb 4 2021

    Organoid News by STEMCELL Technologies

    Vol. 2.04 – 4 February, 2021

    TOP STORY

    A Gene–Environment-Induced Epigenetic Program Initiates Tumorigenesis

    Researchers showed that the combination of Kras mutation and tissue damage promoted a unique chromatin state in the pancreatic epithelium that distinguished neoplastic transformation from normal regeneration and was selected for throughout malignant evolution.
    [Nature]

    Abstract

    Immunofluorescence image of adult stem cell-derived intestinal organoids.

    PUBLICATIONSRanked by the impact factor of the journal

    3D
    Bioprinting of High Cell-Density Heterogeneous Tissue Models through Spheroid Fusion within Self-Healing Hydrogels

    Scientists bioprinted iPSC-derived cardiac microtissue models with spatially controlled cardiomyocyte and fibroblast cell ratios to replicate the structural and functional features of scarred cardiac tissue that arise following myocardial infarction, including reduced contractility and irregular electrical activity.
    [Nature Communications]

    Full Article

    Dual Functions of SPOP and ERG Dictate Androgen Therapy Responses in Prostate Cancer

    The authors showed that mutually exclusive prostate cancer driver alterations involving the ERG transcription factor and the ubiquitin ligase adaptor SPOP were synthetic sick.
    [Nature Communications]

    Full Article

    RINT1
    Regulates SUMOylation and the DNA Damage Response to Preserve Cellular Homeostasis in Pancreatic Cancer

    Investigators proposed RAD50-interacting protein 1 (RINT1) as an essential mediator of cellular homeostasis in pancreatic ductal adenocarcinoma. RINT1 depletion caused severe growth defects in vitro associated with accumulation of DNA double-strand breaks, G2 cell cycle arrest, disruption of Golgi-ER homeostasis, and cell death.
    [Cancer Research]

    Abstract

    A
    Novel Soluble ACE2 Variant with Prolonged Duration of Action Neutralizes SARS-CoV-2 Infection in Human Kidney Organoids

    Human kidney organoids were used for infectivity studies of SARS-CoV-2 in a BSL-3 facility to examine the neutralizing effect of novel ACE2 variants.
    [Journal of the American Society of Nephrology]

    Full Article

    Cell-to-Cell
    Adhesion and Neurogenesis in Human Cortical Development: A Study Comparing 2D Monolayers with 3D Organoid Cultures

    The authors compared transcriptome and cellular phenotypes between telencephalic organoids and monolayers generated in parallel from three biologically distinct iPSCs.
    [Stem Cell Reports]

    Full Article

    Gene
    Therapy of Dominant CRX-Leber Congenital Amaurosis using Patient Stem Cell-Derived Retinal Organoids

    Researchers established an in vitro model of CRX-Leber congenital amaurosis in retinal organoids that showed defective photoreceptor maturation by histology and gene profiling, with diminished expression of visual opsins.
    [Stem Cell Reports]

    Full ArticleGraphical Abstract

    Cdk5rap3 Is Essential for Intestinal Paneth Cell Development and Maintenance

    Paneth cell-specific knockout of Cdk5rap3 caused partial loss of Paneth cells, while inducible acute deletion of Cdk5rap3 resulted in disassembly of the rough endoplasmic reticulum and abnormal zymogen granules in the mature Paneth cells, as well as loss of Paneth cells.
    [Cell Death & Disease]

    Full Article

    Generation
    of Human-Induced Pluripotent Stem Cell-Derived Functional Enterocyte-Like Cells for Pharmacokinetic Studies

    Scientists demonstrated the generation of enterocyte-like cells by culturing human iPSC-derived intestinal progenitor cells on a collagen vitrigel membrane.
    [Stem Cell Reports]

    Full Article

    Organoid
    Cultures Derived from Patients with Papillary Thyroid Cancer

    Investigators describe a three-dimensional culture system for the long-term expansion of patient-derived oapillary thyroid cancer organoid lines.
    [Journal of Clinical Endocrinology & Metabolism]

    Abstract

    Modeling
    Colorectal Tumorigenesis Using the Organoids Derived from Conditionally Immortalized Mouse Intestinal Crypt Cells (ciMICs)

    The authors showed that the ciMIC cells maintained long-term proliferative activity under two-dimensional niche factor-containing culture condition, retained the biological characteristics of intestinal epithelial stem cells, and could form intestinal organoids in three-dimensional culture.
    [Genes & Diseases]

    Abstract
    Full Article

    Gene
    Editing Correction of a Urea Cycle Defect in Organoid Stem Cell Derived Hepatocyte-like Cells

    Researchers demonstrated a practical, and widely applicable approach that included the successful reprogramming of somatic cells from a patient with a urea cycle defect, their genetic correction and differentiation into hepatic organoids.
    [International Journal of Molecular Sciences]

    Full Article

    Inhibition
    of Mitochondrial Function by Metformin Increases Glucose Uptake, Glycolysis and GDF-15 Release from Intestinal Cells

    Investigators used intestinal organoids to explore the effects of metformin on intestinal cell physiology.
    [Scientific Reports]

    Full Article


    Explore scientific events this February with the Science Events Calendar.

    REVIEWS

    The Evolving Definition of Salivary Gland Stem Cells

    Scientists provide an overview of how recent developments in the adult stem cell research field are changing our thinking of the definition of salivary gland stem cells and their potential plasticity upon damage.
    [npj Regenerative Medicine]

    Full Article

    Lung
    Organoids, Useful Tools for Investigating Epithelial Repair after Lung Injury

    The authors review the recent methods of cultivating lung organoids, applications of lung organoids in epithelial repair after injury, and understanding the mechanisms of epithelial repair investigated using lung organoids.
    [Stem Cell Research & Therapy]

    Full Article

    INDUSTRY AND POLICY NEWS

    3D Retinal Organoid Challenge Awards & Recognition

    The National Eye Institute has awarded a team led by Dr. Maria Natalia Vergara at the Sue Anschutz-Rodgers Eye Center, University of Colorado $60,000 for developing an organoid derived from stem cells engineered to fluoresce. This method allows the organoid to demonstrate the cellular composition more clearly and efficiently.
    [National Eye Institute]

    Press Release

    FEATURED EVENT

    Stanford Diabetes Research Center: Insulin 100th Birthday Celebration

    February 18, 2021
    Virtual


    > See All Events

    JOB OPPORTUNITIES


    Faculty Position – Department of Hematology

    St. Jude Children’s Research Hospital – Memphis, Tennessee, United States

    PhD
    Studentship – Bioengineering and Biotechnologies

    Queen Mary University of London – London, England, United Kingdom

    Postdoctoral
    Researcher – Neuroimmunology

    University of Luxembourg – Luxembourg City, Luxembourg

    Research
    Technician – Stem Cell and Genome Editing

    The University of British Columbia – Vancouver, British Columbia, Canada

    Postdoctoral Researcher – Molecular and Functional Neurobiology

    University of Luxembourg – Belval, Luxembourg

    > See All Jobs

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