Organoid News Volume 2.06 | Feb 18 2021

May 12, 2022

ON 2.06 | Feb 18 2021

Vol. 2.06 – 18 February, 2021

TOP STORY

Reintroduction of the Archaic Variant of NOVA1
in Cortical Organoids Alters Neurodevelopment

To investigate the functional importance of an amino acid change in humans, the authors reintroduced the archaic allele into human induced pluripotent cells using genome editing and then followed their neural development through cortical organoids.
[Science]

Abstract Press Release
Graphical Abstract

Survey Report: Where Is Pluripotent Stem Cell Research Now?

PUBLICATIONSRanked by the impact factor of the journal

Neurodevelopmental
Defects and Neurodegenerative Phenotypes in Human Brain Organoids Carrying Parkinson’s Disease-Linked DNAJC6 Mutations

To study the roles of DNAJC6 in Parkinson’s disease (PD) pathogenesis, researchers used human ESCs with CRISPR-Cas9-mediated gene editing. They showed that DNAJC6 mutations caused key PD pathologic features, i.e., midbrain-type dopamine neuron degeneration, pathologic α-synuclein aggregation, increase of intrinsic neuronal firing frequency, and mitochondrial and lysosomal dysfunctions in human midbrain-like organoid.
[Science Advances]

Full Article

Genomic Aberrations after Short-Term Exposure to Colibactin-Producing E.
coli
Transform Primary Colon Epithelial Cells

Colibactin’s distinct mutational signature was reflected in human colorectal cancer, suggesting a causal link. Scientists investigated its transformation potential using organoids from primary murine colon epithelial cells.
[Nature Communications]

Full Article

Malignant
Subclone Drives Metastasis of Genetically and Phenotypically Heterogenous Cell Clusters through Fibrotic Niche Generation

Researchers performed spleen transplantation experiments using a recently established organoid transplantation model. Mouse intestinal tumor-derived organoids carried various combinations of genetic alterations in driver genes.
[Nature Communications]

Full Article

IL-22
Promotes the Formation of a MUC17 Glycocalyx Barrier in the Postnatal Small Intestine during Weaning

Ileal organoids consisted predominantly of enterocytes interspersed with Muc2-expressing goblet cells, recapitulating in vivo epithelial cell organization
[Cell Reports]

Full Article Graphical Abstract

Macrophage-Derived
EDA-A2 Inhibits Intestinal Stem Cells by Targeting miR-494/EDA2R/β-Catenin Signaling in Mice

In colitis crypts, a notable cytokine–cytokine receptor, miR-494-3p-targeted EDA2R and the ligand EDA-A2, suppressed colonic stemness and epithelial repair by inhibiting β-catenin/c-Myc. In differentiated intestinal epithelial cells, miR-494-3p inhibited macrophage recruitment, M1 activation and EDA-A2 secretion by targeting IKKβ/NF-κB in colitis.
[Communications Biology]

Full Article

Impaired
NEPHRIN Localization in Kidney Organoids Derived from Nephrotic Patient iPS Cells

Scientists established iPSCs from two additional patients with different NPHS1 mutations and examined the podocyte abnormalities in kidney organoids derived from these cells.
[Scientific Reports]

Full Article

Role
of Adenomatous Polyposis Coli in Proliferation and Differentiation of Colon Epithelial Cells in Organoid Culture

Researchers performed genetic analyses of adenomatous polyposis coli using a 3D organoid culture of mouse colon epithelia, which enabled the detailed examination of epithelial properties.
[Scientific Reports]

Full Article

Interleukin-28A
Induces Epithelial Barrier Dysfunction in CD Patient-derived Intestinal Organoids

Crohn’s disease patient-derived intestinal organoids were characterized by differentiation gene markers and then exposed to TNF-α, IFN-γ, IL-1β or LPS, or IL-28A with or without GLPG0634.
[American Journal of Physiology-Gastrointestinal and Liver Physiology]

Abstract

Prostaglandin
F2α and EP2 Agonists, and a ROCK Inhibitor Modulate the Formation of 3D Organoids of Grave’s Orbitopathy Related Human Orbital Fibroblasts

3D organoids were prepared from Grave’s orbitopathy (GO) related human orbital fibroblasts obtained from patients with GO.
[Experimental Eye Research]

Abstract

Download an e-book covering the evolution of organoids, from their development to updated applications and future outlooks.

REVIEWS

Non-Matrigel Scaffolds for Organoid Cultures

Scientists discuss the constraints of matrigel and describe other alternative scaffolds that have been used for organoid cultures.
[Cancer Letters]

Abstract

Medical
Progress: Stem Cells as a New Therapeutic Strategy for COVID-19

The authors discuss the latest discoveries in molecular biology, a platform model of pluripotent stem cells in the SARS-CoV-2 study on 3D animal models and nanoconjugates based on stem cells.
[Stem Cell Research]

Full Article

Current
Progress, Challenges, and Future Prospects of Testis Organoids

Scientists review the current progress in the development of scaffold-based testis organoids while focusing on the scaffolding biomaterials, cell sources, and scaffolding approaches.
[Biology of Reproduction]

Abstract

INDUSTRY AND POLICY NEWS

Cytovia Therapeutics and Cellectis Partner to Develop TALEN^® Gene-Edited iPSC-Derived Natural Killer Cells

Cytovia Therapeutics, Inc., and Cellectis announced that they have entered into a strategic research and development collaboration to develop TALEN^® gene-edited iPSC Natural Killer (NK) and Chimeric Antigen Receptor (CAR)-NK cells.
[Cytovia Therapeutics, Inc]

Press Release

FEATURED EVENT

Reintroduction of the Archaic Variant of NOVA1
in Cortical Organoids Alters Neurodevelopment

Neurodevelopmental
Defects and Neurodegenerative Phenotypes in Human Brain Organoids Carrying Parkinson’s Disease-Linked DNAJC6 Mutations

Genomic Aberrations after Short-Term Exposure to Colibactin-Producing E.
coli
Transform Primary Colon Epithelial Cells

Malignant
Subclone Drives Metastasis of Genetically and Phenotypically Heterogenous Cell Clusters through Fibrotic Niche Generation

IL-22
Promotes the Formation of a MUC17 Glycocalyx Barrier in the Postnatal Small Intestine during Weaning

Macrophage-Derived
EDA-A2 Inhibits Intestinal Stem Cells by Targeting miR-494/EDA2R/β-Catenin Signaling in Mice

Impaired
NEPHRIN Localization in Kidney Organoids Derived from Nephrotic Patient iPS Cells

Role
of Adenomatous Polyposis Coli in Proliferation and Differentiation of Colon Epithelial Cells in Organoid Culture

Interleukin-28A
Induces Epithelial Barrier Dysfunction in CD Patient-derived Intestinal Organoids

Prostaglandin
F2α and EP2 Agonists, and a ROCK Inhibitor Modulate the Formation of 3D Organoids of Grave’s Orbitopathy Related Human Orbital Fibroblasts

Non-Matrigel Scaffolds for Organoid Cultures

Medical
Progress: Stem Cells as a New Therapeutic Strategy for COVID-19

Current
Progress, Challenges, and Future Prospects of Testis Organoids

Cytovia Therapeutics and Cellectis Partner to Develop TALEN^® Gene-Edited iPSC-Derived Natural Killer Cells

Cambridge
Cancer Epigenetics Club: Dirk Schübeler

> See All Events

Postdoctoral
Research Fellow – Scaffold-Based 3D Model of Neurodegeneration

Postdoctoral Position – Hematopoietic Development

Postdoctoral Researcher – 3D In Vitro Systems for Respiratory Bacterial Infections

Faculty Position – Department of Hematology

Research
Technician – Stem Cell and Genome Editing

> See All Jobs