Organoid News Volume 2.08 | Mar 4 2021

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    ON 2.08 | Mar 4 2021


    Organoid News by STEMCELL Technologies
    Vol. 2.08 – 4 March, 2021
    TOP STORY

    MAP3K2-Regulated Intestinal Stromal Cells Define a Distinct Stem Cell Niche

    To determine the physiological role of MAP3K2-regulated intestinal stromal cells (MRISCs), scientists co-cultured MRISCs with colonic organoids and found that organoid growth was greater than when co-cultured with MRISC-depleted stromal cells
    [Nature]

    Abstract

    Watch the Webinar On-Demand: “Re-Creating Disease with Kidney Organoids and CRISPR-Cas9”
    PUBLICATIONSRanked by the impact factor of the journal

    Somatic Mutations and Single-Cell Transcriptomes Reveal the Root of Malignant Rhabdoid Tumors

    Investigators studied the origin of malignant rhabdoid tumor by combining phylogenetic analyses and single-cell mRNA studies in patient-derived organoids.
    [Nature Communications]

    Full Article

    Differential Regulation of β-Catenin-Mediated Transcription via N- and C-Terminal Co-factors Governs Identity of Murine Intestinal Epithelial Stem Cells

    Scientists took advantage of knock-in mice harboring transgenic β-catenin alleles with mutations that specifically impaired the recruitment of N- or C-terminal transcriptional co-factors.
    [Nature Communications]

    Full Article

    Evidence That Polyploidy in Esophageal Adenocarcinoma Originates from Mitotic Slippage Caused by Defective Chromosome Attachments

    The authors investigated the origin of polyploidy in esophageal adenocarcinoma, a highly heterogenous cancer, using a combination of genomics and cell biology approaches in EAC cell lines, organoids, and tumors.
    [Cell Death & Differentiation]

    Full Article

    A Self-Healing, Antioxidative Organoid-Chip for Cell Sorting, Capture and Release-on-Demand

    Researchers developed a facile organoid chip composed of the adhesive film prepared via layer-by-layer self-assembly and the super-lubricating film polymerized under ultraviolet light.
    [Chemical Engineering Journal]

    AbstractGraphical Abstract

    The In Vitro Multilineage Differentiation and Maturation of Lung and Airway Cells from Human Pluripotent Stem Cell–Derived Lung Progenitors in 3D

    Investigators describe a strategy for directed differentiation of hPSCs into mature lung and airway epithelial cells obtained through maturation of NKX2.1+ hPSC-derived lung progenitors in a 3D matrix of collagen I in the absence of glycogen synthase kinase 3 inhibition.
    [Nature Protocols]

    Abstract

    Transcriptional Networks Identify Synaptotagmin-Like 3 as a Regulator of Cortical Neuronal Migration during Early Neurodevelopment

    Synaptotagmin-like 3-knockout caused an abnormal distribution of deep-layer neurons in brain organoids and reduced presynaptic neurotransmitter release in human embryonic stem cell-derived neurons.
    [Cell Reports]

    Full ArticleGraphical Abstract

    Tektin4 Loss Promotes Triple-Negative Breast Cancer Metastasis through HDAC6-Mediated Tubulin Deacetylation and Increases Sensitivity to HDAC6 Inhibitor

    The authors found that tektin4 loss sensitized triple-negative breast cancer cells, xenograft models, and patient-derived organoid models to the HDAC6-selective inhibitor ACY1215.
    [Oncogene]

    Abstract

    The Protein Phosphatase PPM1A Dephosphorylates and Activates YAP to Govern Mammalian Intestinal and Liver Regeneration

    Genetic ablation or depletion of protein phosphatase magnesium-dependent 1 A (PPM1A) in cells, organoids, and mice elicited an enhanced Yes-associated protein (YAP)/TAZ cytoplasmic retention and resulted in the diminished cell proliferation, severe gut regeneration defects in colitis, and impeded liver regeneration upon injury.
    [PLoS Biology]

    Full Article

    Volume-Constrained Microcontainers Enable Myoepithelial Functional Differentiation in Highly Parallel Mammary Organoid Culture

    Investigators enclosed organoids in nanoliter-scale, topologically enclosed, fluid compartments surrounded by agar. Organoids cultured in these “microcontainers” appeared to secrete enough extracellular matrix to yield a self-sufficient microenvironment without exogenous supplements.
    [iScience]

    AbstractGraphical Abstract

    Characterization of Mitochondrial Health from Human Peripheral Blood Mononuclear Cells to Cerebral Organoids Derived from Induced Pluripotent Stem Cells

    Researchers developed cerebral organoids from induced pluripotent stem cells derived from human peripheral blood mononuclear cells and monitored mitochondrial health from the primary, reprogrammed and differentiated stages.
    [Scientific Reports]

    Full Article

    Impact of Hypoxia and Ampk on Cftr-Mediated Bicarbonate Secretion in Human Cholangiocyte Organoids

    Scientists investigated the effect of hypoxia on cystic fibrosis transmembrane conductance regulator activity in intrahepatic cholangiocyte organoids and test drug-interventions to restore bicarbonate secretion.
    [American Journal of Physiology-Gastrointestinal and Liver Physiology]

    Abstract

    Explore scientific events this March with the Science Events Calendar.
    REVIEWS

    Hepatobiliary Organoids and Their Applications for Studies of Liver Health and Disease: Are We There Yet?

    The authors summarize currently available adult cell‐derived hepatobiliary organoid models and their applications.
    [Hepatology]

    Abstract

    SARS-CoV-2 Infection and Disease Modelling Using Stem Cell Technology and Organoids

    Scientists describe the basic virology and pathogenesis of SARS-CoV-2, highlighting how stem cell technology and organoids can contribute to the understanding of SARS-CoV-2 cell tropisms
    [International Journal of Molecular Sciences]

    Full Article

    INDUSTRY AND POLICY NEWS

    AgeX Therapeutics And LyGenesis To Negotiate Merger Agreement

    AgeX Therapeutics, Inc. and LyGenesis, Inc. announced that they will proceed to negotiate an agreement for a merger of the two companies.
    [AgeX Therapeutics, Inc.]

    Press Release

    FEATURED EVENT

    Stem Cell-Based Embryo Models

    April 13 – May 11, 2021
    Virtual

    > See All Events

    JOB OPPORTUNITIES

    Postdoctoral Position – Single-Cell Dynamics in Intestinal Organoids

    AMOLF – Amsterdam, Netherlands

    Postdoctoral Fellow – Genomics of Endocrine Traits

    Children’s Hospital of Philadelphia – Philadelphia, Pennsylvania, United States

    Postdoctoral Position – Hematopoietic Development

    Icahn School of Medicine at Mount Sinai – New York, New York, United States

    Postdoctoral Researcher – 3D In Vitro Systems for Respiratory Bacterial Infections

    NCCR AntiResist – Basel, Switzerland

    Research Technician – Stem Cell and Genome Editing

    The University of British Columbia – Vancouver, British Columbia, Canada

    > See All Jobs

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