DIABETES Mutual Effect of Subcutaneously Transplanted Human Adipose-Derived Stem Cells and Pancreatic Islets within Fibrin Gel Researchers evaluated whether fibrin gel with human adipose-derived stem cells (hADSCs) and rat pancreatic islets could cure diabetes mellitus when transplanted into the subcutaneous space of diabetic mice. The hADSCs did not directly differentiate into endothelial cells, but showed evidence of differentiation toward insulin-secreting cells. [Biomaterials] Abstract Endoplasmic Reticulum Stress Sensitizes Pancreatic Beta Cells to Interleukin-1ß-Induced Apoptosis via Bim/A1 Imbalance Scientists investigated whether mild endoplasmic reticulum (ER) stress also sensitizes beta cells to cytokine-induced apoptosis. Cyclopiazonic acid-induced ER stress enhanced the interleukin-1ß apoptosis in INS-1E and primary rat beta cells. This was not prevented by XBP1 knockdown, indicating the dissociation between the pathways leading to inflammation and cell death. [Cell Death Dis] Full Article Pten Controls ß-Cell Regeneration in Aged Mice by Regulating Cell Cycle Inhibitor p16ink4a Tissue regeneration diminishes with age, concurrent with declining hormone levels including growth factors such as insulin-like growth factor-1. Scientists investigated the molecular basis for such decline in pancreatic ß-cells where loss of proliferation occurs early in age, and is proposed to contribute to the pathogenesis of diabetes. They studied the regeneration capacity of ß-cells in mouse model where PI3K/AKT pathway downstream of insulin/insulin-like growth factor-1 signaling, is upregulated by genetic deletion of Pten (phosphatase and tensin homologue deleted on chromosome 10) specifically in insulin producing cells. [Aging Cell] Abstract Global Gene Expression Profiling of Pancreatic Islets in Mice during Streptozotocin-Induced ß-Cell Damage and Pancreatic Glp-1 Gene Therapy Researchers analyzed the mechanisms of streptozotocin (STZ)-induced diabetes and glucagon-like peptide-1-(glp-1)-mediated ß-cell protection in STZ-treated mice. One week after multiple low-dose STZ administrations, pancreatic ß-cells showed impaired insulin expression, while maintaining expression of nuclear Nkx6.1. [Dis Model Mech] Abstract | Full Article Blockage of Progesterone Receptor Effectively Protects Pancreatic Islet Beta Cell Viability Investigators found that progesterone receptor (PR) activation increased the pro-inflammatory cytokines (PIC)-induced injury in Min6 cells, and PR blockage with siRNA interference protected the cells from damage. Moreover, the new discovered PR antagonist SC51089 effectively improved cell survival by reducing the PIC-stimulated cell apoptosis in Min6 cells. [Steroids] Abstract PANCREATIC CANCER PARP-1 Regulates Resistance of Pancreatic Cancer to TRAIL Therapy Researchers aimed to identify combination therapies that enhance the efficacy of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) therapy; and investigated the underlying mechanisms. Poly(ADP-ribose) polymerase-1 (PARP-1) was found highly expressed in the TRA-8-resistant PANC-1 and Suit-2 cells, compared with TRA-8-sensitive BxPc-3 and MiaPaca-2. [Clin Cancer Res] Abstract Clinical Impact of Pentraxin Family Expression on Prognosis of Pancreatic Carcinoma Using pancreatic carcinoma cell lines and gene transfectant, scientists measured long pentraxin (PTX3) level in culture solution and carried out cellular migration assay in vitro. Elevated PTX3 production was observed in several cell lines, and a direct relationship between migratory activity and PTX3 level was identified in vitro. [Brit J Cancer] Abstract Profilin1 Sensitizes Pancreatic Cancer Cells to Irradiation by Inducing Apoptosis and Reducing Autophagy Researchers demonstrated how profilin1 affected pancreatic cancer radiosensitivity. Researchers showed profilin1 was down-regulated in pancreatic cancer cells after exposure to radiation, and re-expression of profilin1 suppressed tumor cell viability and increased DNA damage following irradiation. [Curr Mol Med] Abstract miR-210 Regulates the Interaction between Pancreatic Cancer Cells and Stellate Cells Researchers aimed to clarify the role of microRNAs (miRNAs) in the interaction between pancreatic stellate cells (PSCs) and pancreatic cancer cells. miR-210 was identified as an upregulated miRNA by co-culture with PSCs. Conditioned media of PSCs activated ERK and Akt, but not hypoxia-inducible factor-1a pathway. [Biochem Biophys Res Commun] Abstract Silencing of GATA6 Suppresses SW1990 Pancreatic Cancer Cell Growth In Vitro and Up-Regulates Reactive Oxygen Species Investigators studied the role of GATA6 expression on proliferation and apoptosis of pancreatic cancer cells and the role of reactive oxygen species. RNA interference against GATA6 successfully inhibited mRNA and protein expression of GATA6 in the SW1990 pancreatic cancer cell line. [Dig Dis Sci] Abstract |