DIABETES & PANCREATITIS Reporter Islets in the Eye Reveal the Plasticity of the Endocrine Pancreas Researchers describe the application of a natural body window that successfully reports on the properties of in situ pancreatic islets. As proof of principle, they transplanted “reporter islets” into the anterior chamber of the eye of leptin-deficient mice. [Proc Natl Acad Sci USA] Abstract High Glucose Exposure Promotes Activation of Protein Phosphatase 2A in Rodent Islets and INS-1 832/13 β-Cells by Increasing the Posttranslational Carboxylmethylation of Its Catalytic Subunit Scientists report activation of protein phosphatase 2A (PP2A) in normal rat islets and insulin-secreting INS-1 832/13 cells under the duress of hyperglycemic conditions. Small interfering RNA-mediated knockdown of the catalytic subunit of PP2A markedly attenuated glucose-induced activation of PP2A. [Endocrinology] Abstract A Novel Gene Delivery Method Transduces Porcine Pancreatic Duct Epithelial Cells Scientists catheterized the umbilical artery of wild-type newborn pigs and delivered an adeno-associated virus serotype 9 vector expressing green-fluorescent protein or vehicle to the celiac artery, the vessel that supplies major branches to the pancreas. [Gene Ther] Abstract Activation of PPARβ/δ Protects Pancreatic β Cells from Palmitate-Induced Apoptosis by Upregulating the Expression of GLP-1 Receptor Researchers showed that peroxisome proliferator-activated receptor (PPAR)β/δ activation decreased apoptosis in β cells and robustly stimulated glucagon-like peptide-1 receptor (GLP-1R) expression under lipotoxic conditions. GW501516 enhanced glucose-stimulated insulin secretion impaired by palmitate through stimulation of GLP-1R expression in β cells. [Cell Signal] Abstract R-Spondin1 Deficiency Enhances [Beta]-Cell Neogenesis in a Murine Model of Diabetes Scientints hypothesized that R-spondin1 (Rspo1) deficiency enhances [beta]-cell neogenesis in streptozotocin (STZ)-induced diabetes. [beta]-cell mass, islet number, and islet size distribution did not differ between STZ-induced Rspo1+/+ and Rspo1-/- mice, but Rspo1-/- animals had reduced [beta]-cell apoptosis and increased numbers of insulin-positive ductal cells, indicating [beta]-cell neogenesis. [Pancreas] Abstract PANCREATIC CANCER Low CHD5 Expression Activates the DNA Damage Response and Predicts Poor Outcome in Patients Undergoing Adjuvant Therapy for Resected Pancreatic Cancer Researchers evaluated the relationship of CHD5 expression with DNA damage response activation in human pancreatic cancer cells and the association of CHD5 expression in 80 patients with resected pancreatic adenocarcinoma by immunohistochemical analysis with clinical outcome. CHD5 depletion and low CHD5 expression in human pancreatic cancer cells lead to increased H2AX-Ser139 and CHK2-Thr68 phosphorylation and accumulation into nuclear foci. [Oncogene] Abstract Overexpression of Calreticulin Contributes to the Development and Progression of Pancreatic Cancer Scientists studied the clinicopathological significance for Calreticulin expression in pancreatic cancer (PC), and its functional relationship with other signaling genes in regulating the biological behavior of PC cells. [J Cell Physiol] Abstract Histone Deacetylase 6 and Cytoplasmic Linker Protein 170 Function Together to Regulate the Motility of Pancreatic Cancer Cells Researchers found that histone deacetylase 6 (HDAC6), a member of the class II HDAC family, is highly expressed at both protein and mRNA levels in human pancreatic cancer tissues. [Protein Cell] Abstract Heat-Shock Protein 90 Inhibitors Synergistically Enhance Melanoma Differentiation-Associated Gene-7-Mediated Cell Killing of Human Pancreatic Carcinoma Scientists examined possible antitumor effects of adenoviruses expressing melanoma differentiation-associated gene-7/interleukin-24 (Ad-mda-7) and a heat-shock protein 90 (Hsp90) inhibitor to human pancreatic carcinoma cells. Ad-mda-7 and an Hsp90 inhibitor, geldanamycin (GA), produced cytotoxic effects, and a combinatory use of Ad-mda-7 and GA further achieved synergistic effects. [Cancer Gene Ther] Abstract Sineoculis Homeobox Homolog 1 Protein Overexpression as an Independent Biomarker for Pancreatic Ductal Adenocarcinoma To investigate the clinicopathological significance of sineoculis homeobox homolog 1 (SIX1) expression in pancreatic ductal adenocarcinoma (PDAC), and to further identify its role as a potential biomarker and therapeutic target in PDAC, 103 PDAC tissue samples and 45 normal pancreatic tissue samples were immunohistochemically stained for SIX1 protein. The localization of SIX1 protein was detected in Panc-1 cancer cells using immunofluorescence staining. [Exp Mol Pathol] Abstract |