DIABETES & PANCREATITIS GLP-1R-Targeting Magnetic Nanoparticles for Pancreatic Islet Imaging Researchers synthesized an exendin-4 conjugated magnetic iron oxide based nanoparticle probe targeting glucagon-like peptide-1 receptor (GLP-1R), which is highly expressed on the surface of pancreatic beta-cells. In vitro studies in ßTC-6 beta-cell line showed specific accumulation of the targeted probe compared to non-targeted. [Diabetes] Abstract Cdc42/N-WASP Signaling Links Actin Dynamics to Pancreatic ß Cell Delamination and Differentiation Using the developing mouse pancreas as a model system, scientists showed that ß cell delamination and differentiation are two independent events, which are controlled by Cdc42/N-WASP signaling. [Development] Abstract Disulfide Stress: A Novel Type of Oxidative Stress in Acute Pancreatitis Researchers analyzed redox status of thiols and aimed to identify mixed disulfides and targets of redox signaling in pancreas in experimental acute pancreatitis as a model of acute inflammation associated with glutathione depletion. [Free Radic Biol Med] Abstract Autocrine C-Peptide Mechanism Underlying INS1 Beta Cell Adaptation to Oxidative Stress Researchers tested the hypothesis that an autocrine activity of C-peptide similarly decreases reactive oxygen species (ROS) when INS1 beta cells are exposed to stressful conditions of diabetes. C-peptide significantly decreased high glucose- and H2O2-induced ROS and prevented apoptosis of INS1 beta cells. [Diabetes Metab Res Rev] Abstract Contribution of Protein Kinase Ca in the Stimulation of Insulin by the Down-Regulation of Cavß Subunits Examination of protein kinase C (PKC) isozymes in the pancreatic ß-cells of voltage-gated calcium (Cav)ß2- or ß3 small interfering RNA-injected mice showed that three PKC isozymes, viz., PKC a, ßII and ?, translocated to the membrane. [Endocrine] Full Article PANCREATIC CANCER Novel Bispecific Antibodies Increase ?d T-Cell Cytotoxicity against Pancreatic Cancer Cells Researchers demonstrated that ?d T-cells isolated from patients with pancreatic ductal adenocarcinoma tumor infiltrates lyse pancreatic tumor cells after selective stimulation with phosphorylated antigens. [Cancer Res] Abstract FOXM1c Promotes Pancreatic Cancer Epithelial-to-Mesenchymal Transition and Metastasis via Upregulation of Expression of the Urokinase Plasminogen Activator System The effect of altered expression of Forkhead box M1 (FOXM1) and urokinase plasminogen activator receptor on epithelial-to-mesenchymal transition and metastasis was examined using animal models of pancreatic cancer. Pancreatic tumor specimens and cell lines predominantly overexpressed the FOXM1 isoform FOXM1c. [Clin Cancer Res] Abstract Hedgehog Acyltransferase as a Target in Pancreatic Ductal Adenocarcinoma Researchers showed that Hedgehog acyltransferase, the enzyme responsible for the attachment of palmitate onto Sonic hedgehog (Shh), is a novel target for inhibition of Shh signaling in pancreatic cancer cells. [Oncogene] Abstract Tumor-Associated Macrophages Exhibit Pro- and Anti-Inflammatory Properties by Which They Impact on Pancreatic Tumorigenesis Since a detailed analysis of tumor-associated macrophages (TAMs) in human pancreatic ductal adenocarcinoma (PDAC) development is still lacking, freshly isolated PDAC-derived TAMs were analyzed for their phenotype and impact on epithelial-mesenchymal-transition of benign and malignant pancreatic ductal epithelial cells. [Int J Cancer] Abstract MicroRNA-148b and MicroRNA-152 Reactivate Tumor Suppressor Genes through Suppression of DNA Methyltransferase-1 Gene in Pancreatic Cancer Cell Lines Scientists investigated the effect of microRNA (miR)-148b and -152 on reverting the tumorigenic phenotype of pancreatic cancer cell lines. In order to investigate whether miR-148b and -152 are involved in the regulation of DNMT-1, luciferase reporter assay was used and confirmed that the DNMT-1 mRNA could be a target for miR-148b and miR-152. [Cancer Biol Ther] Full Article |