DIABETES & PANCREATITIS Glucagon-Secreting Alpha Cell Selective Two-Photon Fluorescent Probe TP-α: For Live Pancreatic Islet Imaging Through fluorescent image based screening using three pancreatic cell lines, scientists discovered two photon-alpha (TP-α) from a TP fluorescent dye library TP-green. In vitro fluorescence test showed that, TP-α have direct interaction with glucagon with a significant fluorescence increase, but not with insulin or other hormones/analytes. [J Am Chem Soc] Abstract glucagon Is Essential for Alpha Cell Transdifferentiation and Beta Cell Neogenesis Using lineage-tracing assays in a transgenic zebrafish model of beta cell ablation, scientists demonstrated conserved plasticity of alpha cells during islet regeneration. In addition, they showed that glucagon expression is upregulated after injury. [Development] Abstract Peroxisome Proliferator-Activated Receptorβ/δ Activation Is Essential for Modulating p-Foxo1/Foxo1 Status in Functional Insulin-Positive Cell Differentiation Researchers showed a novel role of peroxisome proliferator-activated receptor (PPAR)β/δ activation in determining insulin-positive (INS+) cell differentiation and functional maturation. They found PPARβ/δ expression selectively upregulated in mouse embryonic pancreases or stem cells-derived INS+ cells at the pancreatic mature stage in vivo and in vitro. [Cell Death Dis] Full Article 3,4-Dihydroxyphenylacetic Acid, a Microbiota-Derived Metabolite of Quercetin, Protects against Pancreatic β-Cells Dysfunction Induced by High Cholesterol Investigators determined the protective effect of 3,4 dihydroxyphenylacetic acid against apoptosis, mitochondrial dysfunction and oxidative stress induced by cholesterol in Min6 pancreatic β-cells. [Exp Cell Res] Abstract PANCREATIC CANCER Increased In Vitro and In Vivo Sensitivity of BRCA2-Associated Pancreatic Cancer to the Poly(ADP-Ribose) Polymerase-1/2 Inhibitor BMN 673 Researchers assessed the sensitivities of BRCA2-deficient (Capan-1) and -proficient (MIA PaCa-2) pancreatic ductal adenocarcinoma cell lines to a panel of DNA crosslinking agents (DCLs) and PARP inhibitors (PARPis). Compared to MIA PaCa-2, Capan-1 was significantly more sensitive to all tested DCLs and PARPis, with similar increased sensitivities to cisplatin and the PARPi BMN 673 compared to other DCLs and the PARPi veliparib. [Cancer Lett] Abstract Slug-Dependent Upregulation of L1CAM Is Responsible for the Increased Invasion Potential of Pancreatic Cancer Cells following Long-Term 5-FU Treatment To investigate acquired 5-fluorouracil (5-FU) resistance in pancreatic adenocarcinoma, researchers established chemoresistant monoclonal cell lines from the Panc 03.27 cell line by long-term exposure to increasing doses of 5-FU. [PLoS One] Full Article Amiloride Sensitizes Human Pancreatic Cancer Cells to Erlotinib In Vitro through Inhibition of the PI3K/AKT Signaling Pathway Cell proliferation, colony formation, cell cycle and apoptosis were analyzed in four human pancreatic cancer cell lines Bxpc-3, PANC-1, Aspc-1 and CFPAC-1 treated with erlotinib or amiloride alone, or in their combination. [Acta Pharmacol Sin] Full Article Diverse Effects of LPA4, LPA5 and LPA6 on the Activation of Tumor Progression in Pancreatic Cancer Cells To assess the roles of lysophosphatidic acid (LPA) receptors LPA4, LPA5 and LPA6 in cellular functions of pancreatic cancer cells, scientists generated LPA receptor knockdown cells from PANC-1 cells. [Biochem Biophys Res Commun] Abstract |