Pancreatic Cell News Volume 6.32 | Aug 18 2015

Mast Cells Infiltrate Pancreatic Islets in Human Type 1 Diabetes
Pancreatic tissue from donors without diabetes and with type 1 and 2 diabetes was studied using different microscopy techniques to identify islet-infiltrating cells. The direct effects of histamine exposure on isolated human islets and INS-1E cells were assessed using cell-survival studies and molecular mechanisms. [Diabetologia] Abstract

Tissue Factor/Factor VIIa Signaling Promotes Cytokine-Induced Beta Cell Death and Impairs Glucose-Stimulated Insulin Secretion from Human Pancreatic Islets
Researchers studied the effects of tissue factor (TF)/coagulation factor VIIa signaling on cytokine-induced beta cell death and islet function in vitro. Human pancreatic islets and MIN-6 beta cells were used to study TF mRNA and protein expression using real-time PCR, immunoblotting and flow cytometry. [Diabetologia] Abstract

Inhibition of ZEB1 Expression Induces Redifferentiation of Adult Human β Cells Expanded In Vitro
Downregulation of ZEB1 using shRNA in expanded β-cell-derived cells induced mesenchymal-epithelial transition, β-cell gene expression, and proliferation attenuation. [Sci Rep] Full Article

A Novel GLP-1 Receptor Interacting Protein ATP6ap2 Regulates Insulin Secretion in Pancreatic Beta Cells
ATPase associated protein 6 (ATP6ap2) was shown to be abundant in islets including both alpha and beta cells. When GLP1R and ATP6ap2 were co-expressed in beta cells, GLP1R was shown to directly interact with ATP6ap2, as assessed by co-immunoprecipitation. [J Biol Chem] Abstract | Full Article

PANCREATIC CANCER

Lunatic Fringe Is a Potent Tumor Suppressor in Kras-Initiated Pancreatic Cancer
Scientists showed that Lunatic Fringe (Lfng) is uniquely expressed in a subset of acinar cells in the adult pancreas. Deletion of Lfng in the Kras^LSL-G12D/+;Pdx1-Cre mouse model caused increased activation of Notch3 throughout pancreatic ductal adenocarcinoma initiation and progression, and Notch1 after the onset of disease, associated with marked upregulation of Notch target gene Hes1. [Oncogene] Abstract

High ROR2 Expression in Tumor Cells and Stroma Is Correlated with Poor Prognosis in Pancreatic Ductal Adenocarcinoma
Immunohistochemical analysis with tissue microarrays was used to evaluate RTK-like orphan receptor 2 (ROR2) expression in pancreatic ductal adenocarcinoma and to investigate the relationship of this expression to clinicopathological factors and prognosis. [Sci Rep] Full Article

CDX2 Inhibits Pancreatic Adenocarcinoma Cell Proliferation via Promoting Tumor Suppressor miR-615-5p
Investigators observed that CDX2 expression was much lower in mouse pancreatic adenocarcinoma tissues and pancreatic cancer cells. [Tumor Biol] Abstract

Moxifloxacin and Ciprofloxacin Induces S-Phase Arrest and Augments Apoptotic Effects of Cisplatin in Human Pancreatic Cancer Cells via ERK Activation
Researchers found that both the fluoroquinolones suppressed the proliferation of pancreatic cancer cells by causing S-phase arrest and apoptosis. Blockade in S-phase of cell cycle was associated with decrease in the levels of p27, p21, CDK2, cyclin-A and cyclin-E. [BMC Cancer] Full Article

NF45 Overexpression Is Associated with Poor Prognosis and Enhanced Cell Proliferation of Pancreatic Ductal Adenocarcinoma
Scientists investigated the clinical relevance and potential role of NF45 expression in pancreatic ductal adenocarcinoma (PDAC) development. Western blot analysis revealed that NF45 was remarkably upregulated in PDAC tissues, compared with the adjacent non-tumorous ones. [Mol Cell Biochem] Abstract

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Amaranth Diabetes Foundation Presents $500,000 Donation to the American Diabetes Association Research Foundation to Sponsor Four Promising Projects in Diabetes Research
The American Diabetes Association Research Foundation received a $505,623 donation from the Amaranth Diabetes Foundation (ADF). The ADF is sponsoring four peer-reviewed research projects. [American Diabetes Association] Press Release

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NEW Postdoctoral Position – Metabolic Diseases (ETH Zürich)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

Postdoctoral Research Fellow – Development and Pathogenesis of Pancreatic Cancer (University College London)

PhD Position – Beta-Cell Biology and Diabetes (University of British Columbia)

Postdoctoral Research Assistant – Islet Cell Electrophysiology in Diabetes Research (University of Oxford)

Harry Eagle Scholar Awards for Postdoctoral Candidates (Albert Einstein College of Medicine of Yeshiva University)

Postdoctoral Positions – Cancer Research (Rutgers University)

Postdoctoral Fellowship – Pancreatic Beta Cell Biology and Regeneration, Relating to Diabetes (Mount Sinai School of Medicine)

Postdoctoral Fellowship – Immunopathogenesis of Type 1 Diabetes (Tongji Medical College)

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