DIABETES & PANCREATITIS Postnatal β-Cell Maturation Is Associated with Islet-Specific MicroRNA Changes Induced by Nutrient Shifts at Weaning Investigators showed that β-cell maturation is associated with changes in microRNA expression induced by the nutritional transition that occurs at weaning. When mimicked in newborn islet cells, modifications in the level of specific microRNAs result in a switch in the expression of metabolic enzymes and cause the acquisition of glucose-induced insulin release. [Nat Commun] Full Article Anti-Aging Gene Klotho Attenuates Pancreatic β Cell Apoptosis in Type I Diabetes In vivo β cell-specific expression of mouse Klotho gene (mKL) attenuated β cell apoptosis and prevented streptozotocin-induced diabetes. mKL promoted cell adhesion to collagen IV, increased FAK and Akt phosphorylation, and inhibited caspase 3 cleavage in cultured MIN6 β cells. [Diabetes] Abstract Simvastatin May Induce Insulin Resistance through a Novel Fatty Acid Mediated Cholesterol Independent Mechanism Researchers explored the possible mechanism of statin induced insulin resistance using a well-established cell based model and simvastatin as a prototype statin. Their data showed that simvastatin induces insulin resistance in a cholesterol biosynthesis inhibition independent fashion but does so by a fatty acid mediated effect on insulin signaling pathway. [Sci Rep] Full Article Rapid, High Efficiency Isolation of Pancreatic β-Cells The authors developed a fluorescent exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional mouse pancreatic β-cells. By targeting the glucagon-like peptide-1 receptor with the fluorescent conjugate, β-cells could be quickly isolated by flow cytometry and were >99% insulin positive. [Sci Rep] Full Article PANCREATIC CANCER Characteristics of Tumor Infiltrating Lymphocyte and Circulating Lymphocyte Repertoires in Pancreatic Cancer by the Sequencing of T Cell Receptors T cells from primary tumor and blood of sixteen patients with pancreatic cancer were characterized by deep sequencing. By analyzing the complementary determining region 3 gene sequence, investigators found no significant differences in the T cell receptor repertoires between patients and healthy controls. [Sci Rep] Full Article Breaking Resistance of Pancreatic Cancer Cells to an Attenuated Vesicular Stomatitis Virus through a Novel Activity of IKK Inhibitor TPCA-1 Vesicular stomatitis virus (VSV) is an effective oncolytic virus against most human pancreatic ductal adenocarcinoma (PDAC) cell lines. To better understand the mechanism of resistance, scientists tested a panel of 16 small molecule inhibitors of different cellular signaling pathways, and identified TPCA-1 and ruxolitinib, as strong enhancers of VSV-ΔM51 replication and virus-mediated oncolysis in all VSV-resistant PDAC cell lines. [Virology] Abstract Systemic Administration of Small Interfering RNA Targeting Human Nestin Inhibits Pancreatic Cancer Cell Proliferation and Metastasis Nestin small interfering RNA (siRNA) inhibited the growth, migration, invasion, and sphere-forming ability of the pancreatic cancer cell lines. Pancreatic cancer cells cotreated with gemcitabine and nestin siRNA exhibited lower cell viability than cells treated with a control siRNA, gemcitabine alone, or nestin siRNA alone. [Pancreas] Abstract Secreted Phosphoprotein 24 kD (Spp24) Inhibits Growth of Human Pancreatic Cancer Cells Caused by BMP-2 Investigators report that bone morphogenetic proteins (BMP)-2 can promote the proliferation of the pancreatic tumor cell line, PANC-1. Spp24, a BMP binding protein, did not affect the proliferation of the cells but promoted the apoptosis of the cells in vitro. [Biochem Biophys Res Commun] Abstract |