LABORATORY RESEARCH 14-3-3 Proteins Modulate the ETS Transcription Factor ETV1 in Prostate Cancer Scientists report that all 14-3-3 proteins, with the exception of the tumor suppressor 14-3-3σ, can bind to ETV1 in a condition manner dictated by its prominent phosphorylation site S216. S216 mutation or 14-3-3τ downregulation was sufficient to reduce ETV1 protein levels in prostate cancer cells, indicating that non-σ 14-3-3 proteins protect ETV1 from degradation. Notably, S216 mutation also decreased ETV1-dependent migration and invasion in benign prostate cells. [Cancer Res] Abstract PSA-Responsive and PSMA-Mediated Multifunctional Liposomes for Targeted Therapy of Prostate Cancer Scientists constructed a dual-modified liposome that incorporated prostate specific antigen (PSA)-responsive and prostate-specific membrane antigen (PSMA)-mediated liposomes and potentially offers double selectivity for prostate cancer. The folate moiety binds quickly to PSMA-positive tumors, and the PSA-responsive moiety is cleaved by PSA that was enriched in tumor tissues. [Biomaterials] Abstract miR-221 Promotes the Development of Androgen Independence in Prostate Cancer Cells via Downregulation of HECTD2 and RAB1A Scientists showed that stably overexpressing microRNA (miR)-221 confers androgen independent cell growth in LNCaP by rescuing LNCaP cells from growth arrest at G1 phase due to the lack of androgen. Overexpressing of miR-221 in LNCaP reduced the transcription of a subgroup of androgen-responsive genes without affecting the androgen receptor (AR) or AR-androgen integrity. [Oncogene] Abstract Differential G Protein Subunit Expression by Prostate Cancer Cells and Their Interaction with CXCR5 Researchers examined differences in G protein expression of normal prostate and prostate cancer (PCa) cell lines (LNCaP, C4-2B, and PC3) by western blot analysis. Of the 22 G proteins studied, Galphai1-3, Gbeta1-4, Ggamma5, Ggamma7, and Ggamma10 were expressed by both normal and PCa cell lines. [Mol Cancer] Abstract | Full Article Foxm1 Expression in Prostate Epithelial Cells Is Essential for Prostate Carcinogenesis In a transgenic adenocarcinoma mouse prostate model of prostate cancer, loss of Foxm1 decreased tumor growth and metastasis. Decreased tumorigenesis was associated with decreased tumor cell proliferation and down-regulation of genes critical for proliferation and metastasis, including Cdc25b, Cyclin B1, Plk-1, LOX and Versican. [J Biol Chem] Abstract | Full Article A Short Peptide Derived from gN Helix Domain of FGF8b Suppressing Growth of Human Prostate Cancer Cells The authors first designed and synthesized a gN helix domain derived short peptide based on the fibroblast growth factor (FGF)8b-FGFR structure analysis. The synthetic peptides inhibited proliferation of prostate cancer cell lines including PC-3 and DU-145 cells. [Cancer Lett] Abstract Knockdown of Lipocalin-2 Suppresses the Growth and Invasion of Prostate Cancer Cells Investigators identified shRNA-LCN2 to determine the role of lipocalin-2 (LCN2) in prostate-cancer cell proliferation, migration, and invasion. LCN2 protein and mRNA expression are higher in PC3 and DU145 cells than in LNCaP and 22Rv cells, and prostate cancer tissue correlated significantly with tumor differentiation and Gleason’s grade. [Prostate] Abstract Androgen Receptor Enhances Entosis, a Non-Apoptotic Cell Death, through Modulation of Rho/ROCK Pathway in Prostate Cancer Cells Scientists dissected the mechanism of androgen receptor (AR) signaling related to entosis incidence in prostate cancer (PCa) progression. Two stable PCa cell lines, named LNCaP-ARsi and C4-2-ARsi were established with stably transfected AR-shRNA to knockdown AR mRNA expression in LNCaP and C4-2 cells, respectively. Androgen-DHT could enhance entosis in LNCaP, C4-2 and PC3-AR9 PCa cells in a dose dependent manner. [Prostate] Abstract CLINICAL RESEARCH Prostate Cancer Cell Telomere Length Variability and Stromal Cell Telomere Length as Prognostic Markers for Metastasis and Death The authors hypothesized that alterations in telomere length in the primary cancer would predict risk of progression to metastasis and prostate cancer death. To test this hypothesis, they conducted a prospective cohort study of 596 surgically treated men who participated in the ongoing Health Professionals Follow-up Study. [Cancer Discov] Abstract The Modified Glasgow Prognostic Score in Prostate Cancer: Results from a Retrospective Clinical Series of 744 Patients The authors described the relationship between modified Glasgow Prognostic Score (mGPS) and survival in patients with prostate cancer after adjustment for other prognostic factors. Patients with mGPS of two had poorest five-year and ten-year relative survival, of 32.6% and 18.8%, respectively. [BMC Cancer] Full Article |