LABORATORY RESEARCH TRIB1 Supports Prostate Tumorigenesis and Tumor-Propagating Cell Survival by Regulation of Endoplasmic Reticulum Chaperone Expression Researchers applied a functional genomic approach to the three-dimensional spheroid cell culture model and identified TRIB1, a member of the Trib family of serine/threonine kinase-like proteins, as an essential factor for prostate cancer cell growth and survival. [Cancer Res] Abstract miR-409-3p/-5p Promotes Tumorigenesis, Epithelial to Mesenchymal Transition and Bone Metastasis of Human Prostate Cancer Elevated expression of miR-409-3p/-5p was observed in bone metastatic prostate cancer cell lines and human prostate cancer tissues with higher Gleason scores. [Clin Cancer Res] Abstract Enhanced Enrichment of Prostate Cancer Stem-Like Cells with Miniaturized 3D Culture in Liquid Core-Hydrogel Shell Microcapsules It usually takes approximately 10 days for the widely used conventional approach to enrich cancer stem-like cells (CSCs) through the formation of CSC-containing aggregates. Scientists report the time can be shortened to two days while obtaining prostate CSC-containing aggregates with better quality based on the expression of surface receptor markers, dye exclusion, gene and protein expression, and in vivo tumorigenicity. [Biomaterials] Abstract Prostate Cancer Cells Preferentially Home to Osteoblast-Rich Areas in the Early Stages of Bone Metastasis – Evidence from In Vivo Models Researchers mapped the distribution of three human prostate cancer cell lines (PC3-NW1, LN-CaP, and C4 2B4) colonizing the tibias of athymic mice following intracardiac injection and evaluated their interaction with potential metastatic niches. [J Bone Miner Res] Abstract Chemical Proteomics Identifies Heterogeneous Nuclear Ribonucleoprotein (hnRNP) A1 as the Molecular Target of Quercetin in Its Anti-Cancer Effects in PC-3 Cells Scientists identified hnRNPA1 as a direct target of quercetin. hnRNPA1 was recruited to stress granules after treatment of cells with quercetin for up to 48 hours, and the levels of cellular inhibitor of apoptosis, an IRES-translation dependent protein, were reduced by hnRNPA1 regulation. [J Biol Chem] Abstract | Full Article A Claudin 3 and Claudin 4-Targeted Clostridium perfringens Protoxin Is Selectively Cytotoxic to PSA-Producing Prostate Cancer Cells A modified protoxin was constructed with a tethered ligand attached to the C-terminus connected by a flexible linker containing a PSA-specific protease cleavage site. This engineered protoxin selectively and efficiently lyses PSA-producing prostate cancer cells whereas CLDN3 and CLDN4 positive cells that do not express PSA are resistant to cytolysis. [Cancer Lett] Abstract DNA Aptamer Evolved by Cell-SELEX for Recognition of Prostate Cancer Researchers developed a new aptamer probe Wy-5a against prostate cancer (PCa) cell line PC-3 by cell-SELEX (Systematic Evolution of Ligands by Exponential enrichment) technique. Wy-5a shows high specificity to the target cells with dissociation constants in the nanomolar range, and does not recognize other tested PCa cell lines and other tested tumor cell lines. [PLoS One] Full Article EZH2-Mediated Inactivation of IFN-γ-JAK-STAT1 Signaling Is an Effective Therapeutic Target in MYC-Driven Prostate Cancer Scientists showed that interferon-γ receptor 1 is directly repressed by EZH2 in a MYC-dependent manner and is downregulated in a subset of metastatic prostate cancers. [Cell Rep] Full Article | Graphical Abstract CLINICAL RESEARCH Temporal Patterns of Late Bowel and Bladder Radiotherapy Toxicity in a Randomized Controlled Trial Assessing Duration of Neo-Adjuvant Hormones in Prostate Cancer Researchers assessed the temporal patterns of late gastrointestinal and genitourinary radiotherapy toxicity and resolution rates in a randomized controlled trial assessing duration of neo-adjuvant hormone therapy for localized prostate cancer. [Acta Oncol] Abstract Time to Disease-Related Pain and First Opioid Use in Patients with Metastatic Castration-Resistant Prostate Cancer Treated with Sipuleucel-T Researchers examined the effect of sipuleucel-T on time to disease-related pain and time to first use of opioid analgesics in metastatic castration-resistant prostate cancer (mCRPC) using data pooled from three randomized Phase III studies in men with asymptomatic or minimally symptomatic mCRPC. [Prostate Cancer Prostatic Dis] Abstract |