LABORATORY RESEARCH LEF1 Targeting EMT in Prostate Cancer Invasion Is Regulated by miR-34a Investigators describe the molecular mechanism of microRNA-34a (miR-34a) and lymphoid enhancer-binding factor-1 (LEF1) in cooperatively regulating prostate cancer cell invasion. [Mol Cancer Res] Abstract Hepsin Inhibits CDK11p58 I.E. Activity by Suppressing Unr Expression and eIF-2α Phosphorylation in Prostate Cancer Scientists found that hepsin inhibited the internal ribosome entry site activity and expression of CDK11p58, which is associated with cell cycle progression and pro-apoptotic signaling in prostate cancer. [Cell Signal] Abstract FOXO3a Modulates WNT/β-Catenin Signaling and Suppresses Epithelial-to-Mesenchymal Transition in Prostate Cancer Cells Researchers demonstrate that forkhead box-O (FOXO)3a significantly inhibits the expression β-catenin in prostate cancer cells. The mechanism of inhibiting β-catenin expression involves the FOXO3a-mediated transactivated microRNA-34b/c, which consequently suppressed β-catenin mRNA expression by targeting the untranslated regions of β-catenin. [Cell Signal] Abstract The Potential of Neurotensin Secreted from Neuroendocrine Tumor Cells to Promote Gelsolin-Mediated Invasiveness of Prostate Adenocarcinoma Cells Researchers investigated the interactions between neuroendocrine cells and LNCaP cells and the involvement of gelsolin in contributing to the invasive potential of LNCaP cells. [Lab Invest] Abstract NUSAP1 Expression Is Upregulated by Loss of RB1 in Prostate Cancer Cells NUSAP1 expression is elevated in the RB1-null DU145 prostate cancer cell line, as opposed to LNCaP and PC-3 cell lines. Furthermore, NUSAP1 expression increases upon knockdown of RB1 in prostate cancer cell lines and decreases after knockdown of E2F1. [Prostate] Abstract CYP3A5 Regulates Prostate Cancer Cell Growth by Facilitating Nuclear Translocation of AR Scientists elucidated the mechanism of regulation of androgen receptor (AR) expression by CYP3A5. CYP3A5 siRNA blocked growth of LNCaP and C4-2 cells by 30-60%. [Prostate] Abstract Adiponectin Inhibits VEGF-A in Prostate Cancer Cells Prostate cancer cell lines examined had all lower levels of adiponectin and adiponectin receptor I, compared to normal healthy prostate tissue. Moreover, overexpression of adiponectin in prostate cancer cells decreased production of vascular endothelial growth factor A (VEGF-A), while adiponectin depletion increased VEGF-A. [Tumor Biol] Abstract Oxidized Low-Density Lipoprotein Is Associated with Advanced-Stage Prostate Cancer Scientists investigated the function of oxidized low-density lipoprotein (ox-LDL) in prostate cancer cell lines in vitro. Phosphorylation protein chips were used to analyze cell signaling pathways in ox-LDL-treated PC-3 cells. [Tumor Biol] Abstract Inhibitory Action of Pristimerin on Hypoxia-Mediated Metastasis Involves Stem Cell Characteristics and EMT in PC-3 Prostate Cancer Cells The authors investigated whether pristimerin affects the bone metastasis, stem cell characteristics and epithelial-mesenchymal transition (EMT) of prostate cancer PC-3 cells subjected to hypoxia. [Oncol Rep] Abstract CLINICAL RESEARCH Radium-223 Dichloride: A Novel Treatment Option for Castration-Resistant Prostate Cancer Patients with Symptomatic Bone Metastases The authors reviewed and evaluated the clinical trial efficacy and safety of radium 223 along with its place in therapy in men with castration-resistant prostate cancer. [Ann Pharmacother] Abstract Mutation Detection in Formalin-Fixed Prostate Cancer Biopsies Taken at the Time of Diagnosis Using Next-Generation DNA Sequencing Scientists assessed whether next-generation DNA sequencing can be used to screen prostate cancer for multiple gene alterations in men routinely diagnosed with this disease and/or who are entered into clinical trials. [J Clin Pathol] Abstract |