The NF-κB Inhibitory Proteins IκBα and IκBβ Mediate Disparate Responses to Inflammation in Fetal Pulmonary Endothelial Cells In newborn rats exposed to intra-amniotic LPS, scientists found increased expression of the NF-κB target gene manganese superoxide dismutase (MnSOD) in the pulmonary endothelium. Supporting these in vivo findings, LPS induced NF-κB activation and MnSOD expression in isolated fetal pulmonary arterial endothelial cells. [J Immunol] Abstract Innate Immune Response of Human Alveolar Type II Cells Infected with SARS-Coronavirus Researchers report that cultivation of alveolar type II cells at an air-liquid interface provides primary cultures in which to study the pulmonary innate immune responses to infection with SARS-CoV and to explore possible therapeutic approaches to modulating their innate immune response. [Am J Resp Cell Mol] Abstract Relationship between Bradykinin-Induced Relaxation and Endogenous Epoxy-Eicosanoid Synthesis in Human Bronchi Investigators hypothesized that Bradykinin (BK) may stimulate endogenous epoxy-eicosanoids (EET) production in human bronchi. To test this hypothesis, the relaxing and hyperpolarizing effects of BK and 14,15-EET were quantified on human bronchi, as well as the effects of various enzymatic inhibitors on these actions. [Am J Physiol – Lung C] Abstract Overexpression of Sulf2 in Idiopathic Pulmonary Fibrosis Researchers focused on the role of heparan sulfate (HS) 6-O-endosulfatase (Sulf)2 in modulating TGF-β1 function and the development of pulmonary fibrosis. They found that Sulf2 mRNA was overexpressed in lung samples from human patients with idiopathic pulmonary fibrosis, and Sulf2 protein was specifically localized to the hyperplastic type II alveolar epithelial cells. [Glycobiology] Abstract In Vitro Effects of Aldehydes Present in Tobacco Smoke on Gene Expression in Human Lung Alveolar Epithelial Cells Investigators exposed A549 cells in vitro to aldehydes and non-aldehyde chemicals (nicotine, hydroquinone and 2,5-dimethylfuran) present in tobacco smoke and used microarrays to obtain a global view of the transcriptomic responses. [Toxicol In Vitro] Abstract LUNG CANCER A Cancer Stem Cell Model for Studying Brain Metastases from Primary Lung Cancer Scientists applied previously developed primary solid tumor-initiating cell models to the study of brain metastases from the lung to evaluate the presence of a cancer stem cell population. Patient-derived brain metastases and the NCI-H1915 cell line were cultured as stem-enriching tumorspheres. For the first time, they demonstrated the presence of a stemlike population in brain metastases from the lung. [J Natl Cancer I] Abstract Mouse Model for ROS1-Rearranged Lung Cancer Genetic rearrangement of the ROS1 receptor tyrosine kinase was recently identified as a distinct molecular signature for human non-small cell lung cancer (NSCLC). However, direct evidence of lung carcinogenesis induced by ROS1 fusion genes remains to be verified. Researchers showed that EZR-ROS1 plays an essential role in the oncogenesis of NSCLC harboring the fusion gene. [PLoS One] Full Article Changes in the Secretory Profile of NSCLC-Associated Fibroblasts after Ablative Radiotherapy: Potential Impact on Angiogenesis and Tumor Growth Cancer-associated fibroblasts (CAFs) isolated from freshly resected human lung tumors were exposed to ablative ionizing radiation (AIR) and analyzed for their release of paracrine factors. The researchers concluded that AIR mediates a transformation on the secretory profile of CAFs that could influence the behavior of other cells in the tumor tissue and hence guide to some extent therapeutic outcomes. [Transl Oncol] Abstract Shedding of c-Met Ectodomain Correlates with c-Met Expression in Non-Small Cell Lung Cancer Researchers measured soluble c-Met and c-Met levels in a panel of pre-clinical models and 197 advanced Chinese non-small cell lung cancer patients by enzyme-linked immunosorbent assay and immunohistochemistry, respectively. They found that shedding of soluble c-Met associates with total c-Met amount in pre-clinical models, and that soluble c-Met correlates with both c-Met expression level and tumor size in humans, while high soluble c-Met predicts poorer outcome. [Biomarkers] Abstract |