Influenza A Virus Nucleoprotein Induces Apoptosis in Human Airway Epithelial Cells: Implications of a Novel Interaction between Nucleoprotein and Host Protein Clusterin Scientists observed that nucleoprotein (NP) contributes to influenza A virus (IAV) infection induced cell death and heterologous expression of NP alone can induce apoptosis in human airway epithelial cells. The apoptotic effect of IAV NP was significant when compared with other known proapoptotic proteins of IAV. [Cell Death Dis] Full Article Chemosensory Brush Cells of the Trachea: A Stable Population in a Dynamic Epithelium Tracheal brush cells (BC) are specialized epithelial chemosensors which utilize the canonical taste transduction cascade to detect irritants. To test whether the adult epithelium retains the capacity to generate BCs, tracheal epithelial cells were recovered from adult mice and grown in an air-liquid interface culture. After transition to differentiation conditions, BCs were detected and were 1% of the total cell population by day 14. [Am J Respir Cell Mol Biol] Abstract Alpha-1 Antitrypsin Modulates Lung Endothelial Cell Inflammatory Responses to TNFα Researchers investigated the role of alpha-1 antitrypsin (A1AT) on primary lung microvascular endothelial cell activation by relevant cytokines such as TNFα or IL-1β. Despite initial marked augmentation of TNFα self-induced transcription, A1AT inhibited TNFα receptor 1 upregulation and significantly reduced TNFα secretion, effects that were associated with inhibition of the TNFα converting enzyme activity. [Am J Respir Cell Mol Biol] Abstract A Role of PGT in Regulating PGE2 Release from BEAS-2B Cells in Response to LPS Human bronchial epithelial BEAS-2B cells were treated with lipopolysaccharide (LPS), and prostaglandin transporter (PGT) inhibitors were tested to evaluate contribution of PGT to prostaglandin E2 (PGE2) release by assessing its extracellular concentration and characterizing PGT-mediated PGE2 efflux in Xenopus laevis oocytes. As a result, LPS elevated mRNA expression of a pro-inflammatory cytokine IL-6 and extracellular concentration of PGE2 in human bronchial epithelial BEAS-2B cells. [J Endocrinol] Abstract NOD-Like Receptors Mediated Activation of Eosinophils Interacting with Bronchial Epithelial Cells: A Link between Innate Immunity and Allergic Asthma Researchers elucidated the nucleotide-binding oligomerization domain (NOD)1 and NOD2-mediated activation of human eosinophils, the principal effector cells for allergic inflammation, upon interacting with human bronchial epithelial BEAS-2B cells in allergic asthma. [Cell Mol Immunol] Abstract 12/15-Lipoxygenase Expressed in Non-Epithelial Cells Causes Airway Epithelial Injury in Asthma Investigators showed that 12/15-lipoxygenase(LOX) expressed in nonepithelial cells caused epithelial injury in asthma pathogenesis. While 12/15-LOX overexpression or IL-13 administration to naïve mice showed airway epithelial injury, 12/15-LOX knockout/knockdown in allergic mice reduced airway epithelial injury. [Sci Rep] Full Article LUNG CANCER Steroid Sulphatase and Oestrogen Sulphotransferase in Human Non-Small-Cell Lung Carcinoma Investigators evaluated the immunoreactivity of steroid sulphatase (STS) and oestrogen sulphotransferase (EST) in non-small-cell lung cancer (NSCLC) cases using immunohistochemistry. The function of STS and EST was further demonstrated using NSCLC cell lines. [Brit J Cancer] Abstract Activation of the FGF2-FGFR1 Autocrine Pathway: A Novel Mechanism of Acquired Resistance to Gefitinib in NSCLC Cells Scientists have established a gefitinib-resistant cell line model by long-term exposure to gefitinib. They used originally gefitinib-sensitive lung cancer cell lines, namely PC9 and HCC827. They found that the expressions of both FGFR1 and FGF2 were increased in PC9 gefitinib-resistant cells compared to those in PC9 naïve cells. [Mol Cancer Res] Abstract Investigation of Radiation-Induced Transcriptome Profile of Radioresistant Non-small Cell Lung Cancer A549 Cells Using RNA-seq Using RNA-seq, a massive sequencing-based approach, scientists examined whole-transcriptome alteration in radioresistant non-small cell lung cancer A549 cells under irradiation, and verified significant radiation-altered genes and their chromosome distribution patterns. They found that epithelial-mesenchymal transition, migration and inflammatory processes could be meaningfully related to regulation of radiation responses in radioresistant A549 cells. [PLoS One] Full Article Aldo-Keto Reductase 1C3 May Be a New Radioresistance Marker in Non-Small-Cell Lung Cancer Scientists explored the role of AKR1C3 in the radiosensitivity to X-rays of human non-small-cell lung cancer (NSCLC) cells. A specific small interfering RNA (siRNA) to target the AKR1C3 gene was used. Although AKR1C3 was significantly overexpressed in both radioresistant subclone cells and NSCLC tissues, a specific AKR1C3 siRNA significantly enhanced cell radiosensitivity and was concomitant with decreased expression of this gene. [Cancer Gene Ther] Abstract |