CRISPR/Cas9-Mediated Knockout of Lcn2 in Human Breast Cancer Cell Line MDA-MB-231 Ameliorates Erastin-Mediated Ferroptosis and Increases Cisplatin Vulnerability
Scientists abrogated lipocalin 2 (Lcn2) expression in MDA-MB-231 breast cancer cells using the CRISPR/Cas9 technology and evaluated its effect on cellular proliferation, migration, and ferroptotic cell death.
[British Journal of Pharmacology] In vivo, investigators studied the role of Caspase-11 in several angiogenesis mouse models using Caspase-11 wild-type and knockout mice. In vitro, scientists detected the effects of Caspase-4 on endothelial functions and Notch signaling by depleting or overexpressing Caspase-4 in HUVECs.
[Proceedings of the National Academy of Sciences of the United States of America] Scientists identified the reversible SUMOylation of FGF receptor 1 (FGFR1) as an intrinsic fine-tuned mechanism in coordinating endothelial angiogenic signaling during neovascularization.