Design and Lyophilization of Lipid Nanoparticles for mRNA Vaccine and Its Robust Immune Response in Mice and Nonhuman Primates

Buffer optimization allowed the determination of lyophilization conditions, where lipid nanoparticle-encapsulated mRNA encoding SARS-CoV-2 spike protein could induce robust immunogenicity in mice after one month of storage at 5°C and 25°C.