Successful Engraftment of Bladder Organoids in De-Epithelialized Mouse Colon

Bladder organoids cultured using bladder specimens from enhanced green fluorescent protein transgenic mice were engrafted to replace proximal colon epithelium stripped from an approximately 1 cm long target site in syngeneic wild-type recipient mice by exposure to ethylenediaminetetraacetic acid by infusion and flushing with phosphate buffered saline.