Spatially-Resolved Single-Cell Assessment of Pancreatic Cancer Expression Subtypes Reveals Co-Expressor Phenotypes and Extensive Intra-Tumoral Heterogeneity

To provide a deeper understanding of PDAC subtypes, scientists developed a multiplex immunofluorescence pipeline that quantifies protein expression of six PDAC subtype markers and permited spatially resolved, single-cell interrogation of pancreatic tumors from resection specimens and core needle biopsies.