Systematic Comparison of Culture Media Uncovers Phenotypic Shift of Primary Human Microglia Defined by Reduced Reliance to CSF1R Signaling
Researchers studied the functional and transcriptomic impacts of such media on human microglia (hMGL). They provided a better understanding of hMGL in culture, with observations that diverge from those previously made in rodent microglia.
[Leukemia] Ex vivo treatment of cells from acute myeloid leukemia (AML) mice and AML patients with bromodomain and extra-terminal domain (BET) inhibitors reversed CD8+ T cell exhaustion by restoring proliferative capacity and expansion of the more functional precursor-exhausted T cells.
[Nature Communications] The authors reported that specific deletion of IFNγR in CD8 T cells promoted tumor control in a mouse model of melanoma, and provide evidence of a negative feedback loop whereby IFNγ depletes stem-like T cells to restrict anti-tumor immunity.
[Nature Immunology] Selectively targeting YTHDF2 in tumor-associated macrophages (TAMs) using a Toll-like receptor 9 agonist-conjugated small interfering RNA reprogrammed TAMs toward an antitumoral phenotype, restrained tumor growth, and enhanced the efficacy of PD-L1 antibody therapy.