CD28-CAR-T Cell Activation through FYN Kinase Signaling Rather Than LCK Enhances Therapeutic Performance
Scientists found that LCK-deficient CAR-T cells were strongly signaled through CAR and had better in vivo efficacy with reduced exhaustion phenotype, as well as enhanced induction of memory and proliferation.
[Nature Communications] Using in vitro stem cell differentiation and loss of function experiments combined with high-throughput genome-wide bisulfite-, bulk-, and single cell RNA-sequencing, researchers dissected the specific role of DNMT3B in cell fate.
[Nature Communications] Using the FUCCI reporter, scientists developed a cell-cycle synchronized hPSC differentiation system for uncovering epigenome and transcriptome dynamics during the first two divisions leading to definitive endoderm.
[Science Advances] Investigators identified an uncharacterized long noncoding RNA NONMMUT028956 (Lnc956) predominantly expressed in mESCs. Lnc956 was accumulated on replication forks to prevent fork collapse and preserve genomic stability and was essential for mouse embryogenesis.