Dynamic Regulation of Airway Surface Liquid pH by TMEM16A and SLC26A4 in Cystic Fibrosis Nasal Epithelia with Rare Mutations

Scientists developed an efficient CRISPR-Cas9-based approach to knock-out relevant transporters in primary airway basal cells lacking CFTR, and then measured dynamic changes in airway surface liquid pH under thin-film conditions in fully differentiated airway cultures.