Generation of an NCS1 Gene Knockout Human Induced Pluripotent Stem Cell Line Using CRISPR/Cas9

Researchers generated a NCS1 knockout human hiPSC line using CRISPR-Cas9 genome editing. It showed regular expression of pluripotent markers, normal iPSC morphology and karyotype as well as no detectable off-target effects on top 6 potentially affected genes.