PIEZO1-Halotag hiPSC Lines: A New Tool to Assay PIEZO1 Localization and Activity from Single Cells to Tissue Organoids

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Scientists used CRISPR engineering to fuse a HaloTag domain to endogenous PIEZO1 in a hiPSC line. As a control, they generated isogenic PIEZO1-HaloTag knockout hiPSCs.
[Biophysical Journal]
Abstract