Efficient Hepatocyte Differentiation of Primary Human Hepatocyte-Derived Organoids Using Three Dimensional Nanofibers (HYDROX) and Their Possible Application in Hepatotoxicity Research

Researchers attempted to culture human liver organoids established from cryopreserved primary human hepatocytes (PHH-derived organoids), using HYDROX, a chemically defined 3D nanofiber. While the proliferative capacity of PHH-derived organoids was lost by HYDROX-culture, the gene expression levels of drug-metabolizing enzymes were significantly improved.