Analysis of Three Characterization Assays Reveals ddPCR of LIN28A as the Most Sensitive for the Detection of Residual Pluripotent Stem Cells in Cellular Therapy Products

Investigators compared three in vitro assays: qPCR, ddPCR, and RT-LAMP using spike-in samples from three independent experiments, each spiked with different PSC lines into a background of primary fibroblasts, to determine their detection limit in measuring a commonly used PSC marker, LIN28A.