Patient-Derived Tumor Organoids Combined with Function-Associated ScRNA-Seq for Dissecting the Local Immune Response of Lung Cancer

Patient-derived primary lung cancer organoids (pLOCs) were generated by isolating tumor cell clusters, including the infiltrated immune cells. A function-associated single-cell RNA sequencing platform allowing both phenotypic evaluation and scRNA-seq at single-organoid level is developed to dissect the tumor immune microenvironment of individual pLCOs.