LSD1/KDM1A and GFI1B Repress Endothelial Fate and Induce Hematopoietic Fate in Induced Pluripotent Stem Cell-Derived Hemogenic Endotheliums

The authors examined the role of the transcriptional factor GFI1B and its co-factor LSD1/KDM1A in endothelial-to-hematopoietic transition. They employed patient-derived iPSC lines with a dominant negative dysfunctional GFI1BQ287* and irreversible pharmacological LSD1/KDM1A inhibition in healthy iPSC lines.