Targeting the PI3K/AKT/mTOR Pathway Offer a Promising Therapeutic Strategy for Cholangiocarcinoma Patients with High Doublecortin-Like Kinase 1 Expression

The authors established doublecortin-like kinase 1 (DCLK1) knockout and DCLK1 overexpression cell lines for Colony Formation Assay and Transwell experiments to explore the tumor-promoting role of DCLK1.