Hyperglycemic Environments Directly Compromise Intestinal Epithelial Barrier Function in an Organoid Model and Hyaluronan (∼35 kDa) Protects via a Layilin Dependent Mechanism

Intestinal organoids derived from mouse colon were grown in normal glucose media (5 mM) or high glucose media (25 mM) to study the impact of hyperglycemic stress on the intestinal barrier.