Automated Live-Cell Single-Molecule Tracking in Enteroid Monolayers Reveals Transcription Factor Dynamics Probing Lineage-Determining Function

To probe transcription factor function by measuring their dynamics during adult intestinal homeostasis, researchers established highly inclined and laminated optical sheet-illumination-based live-cell single-molecule tracking in mouse small intestinal enteroid monolayers recapitulating tissue differentiation hierarchies in vitro.