PNPLA3(148M) Is a Gain-of-Function Mutation That Promotes Hepatic Steatosis by Inhibiting ATGL-Mediated Triglyceride Hydrolysis

The authors quantified and compared the physical interactions between α/β hydrolase domain containing protein 5 and patatin-like phospholipase domain-containing protein 3 (PNPLA3)/adipose TG lipase (ATGL) in cultured hepatocytes using NanoBiT complementation assays and immunocytochemistry.