Specification of Human Brain Regions with Orthogonal Gradients of WNT and SHH in Organoids Reveals Patterning Variations across Cell Lines

The authors designed the Dual Orthogonal-Morphogen Assisted Patterning System (Duo-MAPS) diffusion device to expose spheres of iPSCs to concomitant orthogonal gradients of a posteriorizing and a ventralizing morphogen, activating WNT and SHH signaling, respectively.