Senescence and inflammation are unintended adverse consequences of CRISPR-Cas9/AAV6-mediated gene editing in hematopoietic stem cells

Researchers uncovered a durable senescence-like response in genetically engineered hematopoietic stem and progenitor cells triggered by p53 and interleukin-1/nuclear factor κB activation, which restricts graft size and clonal diversity in long-term transplantation assays.