Spatio-Temporal Calcium Signaling Patterns Underlying Opposing Effects of Histamine and TAS2R agonists in Airway Smooth Muscle

The authors investigated the spatio-temporal dynamics of Ca²⁺ signaling in airway smooth muscle (ASM) cells using single-cell microscopy and deep learning-based segmentation, integrating the results into a comprehensive model of ASM ion channel dynamics to compare the effects of histamine, CQ, and flufenamic acid.