An Overview on In Vivo Generation of CAR-T Cells Using CRISPR-Loaded Functionalized Nanocarriers for Treating B Cell Lineage Acute Lymphoblastic Leukemia.

The authors describe nanoparticles engineered to specifically target blood circulating T lymphocytes and deliver CRISPR/Cas9 complexes that can integrate chimeric antigen receptor constructs into the TRAC locus and knock out immune checkpoint genes like PD-1 simultaneously.