Generation of hiPSC-Derived Brain Microvascular Endothelial Cells Using Directed Differentiation and Transcriptional Reprogramming

Investigators combined directed differentiation of human iPSCs into BBB-primed endothelial cells with overexpression of FOXF2 and ZIC3, transcription factors critical for BMEC identity, to generate reprogrammed BMECs from 3 iPSC lines.