Distinct SOX9 Single-Molecule Dynamics Characterize Adult Differentiation and Fetal-Like Reprogrammed States in Intestinal Organoids

Using automated live-cell single-molecule tracking in intestinal organoid models, scientists revealed an expression-level-independent decrease in the fraction of immobile sex-determining region Y box 9 (SOX9) molecules during differentiation from ∼48% to ∼38%, largely dependent on DNA binding.